🚀 go-pugleaf

Dear Worm breeders,

We have generated a large number of transgenic worms in an unc-119  
background using microparticle bombardment. We need large numbers of  
these transgenics and have been growing them in liquid culture to  
reach the density required. A few months ago, these strains were  
growing really well with a quick generation time. More recently, the  
same strains have become extremely slow growing and have not been  
reaching the densities needed.

We have tried starting fresh cultures from frozen stocks, making  
fresh culture reagents etc, but nothing as improved the growth of  
these strains. The same strains have also not grown well when we have  
put them on solid media.

Has anyone had any experience with the long-term liquid culture of  
transgenics?? Is it perhaps the time of year, as it is now winter in  
the Southern hemisphere!

Any help is appreciated.

Thanks in advance.

Julie-Anne


Julie-Anne Fritz, Ph.D.
Nematode Molecular Genetics Group
The School of Biochemistry & Molecular Biology
Faculty of Science
The Australian National University
Canberra, A.C.T., Australia 0200

Ph(office):   	+ 61 2 6125 4941
Ph(lab):		+ 61 2 6125 5012
Fax: 		+ 61 2 6125 0313
julie-anne.fritz@anu.edu.au