Single worm reverse-transcriptase (RT) PCR

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1 total messages Started by g.Joshua Fri, 11 Sep 2009 11:20

#219

Author: g.Joshua
Date: Fri, 11 Sep 2009 11:20

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Dear colleagues,
I'm advised that putative positives from RNA-interference (RNAi) experiments (using feeding strains from Geneservice {Fraser et al [2000]. Nature 408 (6810) 325-30}) can be checked by RT-PCR. This requires reverse transcriptase reaction on single worms. 
Single worm PCR lysis buffer doesn't seem amenable to reverse transcriptase reaction {?} 
Does anyone have a method/protocol for single worm RT-PCR ?
G. Joshua.
 
 
G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel:     (+44) (0)20 7927 2028
FAX:    (+44) (0)20 7637 4314
E-mail:  g.joshua@lshtm.ac.uk

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