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Article #110Re: Lung fibroblast methotrexate toxicity
From: PiLS
Date: Thu, 12 Jul 2012 20:53
Date: Thu, 12 Jul 2012 20:53
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Sonal Patel <stylosweetu@gmail.com> � plopp�: > I am trying to find out effect of methotrexate on lung fibroblast WT cells > and KO where gene of my interest is knocked out . After 3 weeks on staining > with crystal violet I get lot of background cells in KO alongwith colonies. > Can anyone suggest me how to remove the background cells. Hello, can you provide more info about what you are trying to achieve? Are you using the MTX as a selection agent or are you trying to study its effects on KO cells? In general if you have incomplete selection (assuming that's your problem) reducing the concentration of serum and/or increasing the concentration of the selection agent gives good results. -- PiLS
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From: PiLS <pils@invalid.ca>
Newsgroups: bionet.cellbiol
Subject: Re: Lung fibroblast methotrexate toxicity
Date: Thu, 12 Jul 2012 20:53:41 +0000 (UTC)
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