DNA-damage-response by UV irradiation

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1 total messages Started by Bianca Schäfer Tue, 21 May 2013 15:30

#129

Author: Bianca Schäfer
Date: Tue, 21 May 2013 15:30

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Dear cell-biology-specialists, 

 

I am looking for a protocol to induce DNA-damage-response in cell lines
(f.e. HEK293). 

We would like to use the equivalent of 35 J/m², but we don`t have a
Stratalinker and wonder whether we can use a simple UV-lamp/table usually
used for Ethidiumbromide-geles in our lab. 

We already know, that the media should be removed for the time of the
irradiation. But we didn`t find any information about how long the cells
should be treated with UV. 

 

Many thanks for your help J 

Best Regards!

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