Thread View: bionet.cellbiol
1 messages
1 total messages
Started by Javier Casas
Wed, 02 Feb 2011 00:51
Calcium Crimson ?
Author: Javier Casas
Date: Wed, 02 Feb 2011 00:51
Date: Wed, 02 Feb 2011 00:51
30 lines
3681 bytes
3681 bytes
Hello, Any luck with your Calcium Crimson work? because Iļæ½m having the same problems in aT cell hybridoma. Thanks Hi all. I'm working with Calcium Crimson dye (Molecular Probe) to monitor changes in calcium concentration in COS cells. When I tried to observe the Calcium Crimson-loaded cells with Confocal Laser Scanning Microscope (Bio-Rad), I noticed that not all the cells are readily labeled with Calcium Crimson, which is quite different from my previous experiments with other calcium indicators like Fluo-3 etc. Even worse, the few Calcium Crimson labeled cells appeared to be non-viable by microscopic observation. Is Calcium Crimson is somewhat more toxic than other fluorescent calcium indicators ? Also I'd like to know the appropriate labeling period and working concentration of Calcium Crimson. Any of your kind help would be greatly appreciated, Javier Casas, Ph.D. Department of Immunology and Microbial Science The Scripps Research Institute 10550 North Torrey Pines Rd. La Jolla, CA 92037, USA (858) 784-8184 (lab) (858) 784-9272 (FAX)
Thread Navigation
This is a paginated view of messages in the thread with full content displayed inline.
Messages are displayed in chronological order, with the original post highlighted in green.
Use pagination controls to navigate through all messages in large threads.
Back to All Threads