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Article #118Cell panning
From: Robert Burns
Date: Wed, 22 May 2002 16:12
Date: Wed, 22 May 2002 16:12
23 lines
2561 bytes
2561 bytes
Hi, I am looking for anyone with experience of "panning" techniques using antigens, for the selection of enriched murine splenocyte populations prior to cell fusions for hybridoma production. Any info anybody on workers using this technique? Thanks Robert Robert Burns Antibody Unit Scottish Agricultural Science Agency 82 Craigs Road Edinburgh EH12 8NJ Robert.Burns@sasa.gsi.gov.uk ---
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,277ba360874f69dd,start X-Google-Attributes: gid10eaff,public X-Google-ArrivalTime: 2002-05-22 09:12:37 PST Path: arc1-ca.pugleaf.net!archiver1.google.com!news1.google.com!newsfeed.stanford.edu!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!nycmny1-snh1.gtei.net!news.gtei.net!bloom-beacon.mit.edu!hgmp.mrc.ac.uk!net.bio.net From: Robert.Burns@sasa.gsi.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: Cell panning Date: 22 May 2002 17:12:35 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 24 Sender: mod-diag@hgmp.mrc.ac.uk Approved: BIOSCI Approval Key <biosci-approval-key@net.bio.net> Message-ID: <C39747D75908D31195EE0090273C23E9CB98B1@barra> NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" X-Trace: niobium.hgmp.mrc.ac.uk 1022083956 6456 193.62.192.80 (22 May 2002 16:12:36 GMT) X-Complaints-To: news@net.bio.net NNTP-Posting-Date: Wed, 22 May 2002 16:12:36 +0000 (UTC) X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1CD0117C50 for <diagnost@net.bio.net>; Wed, 22 May 2002 17:08:00 +0100 (BST) X-Received: from mail6.gsi.gov.uk (gateway101.gsi.gov.uk [212.137.57.25]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CB5B717AA3 for <diagnost@net.bio.net>; Wed, 22 May 2002 17:07:56 +0100 (BST) X-Received: from sasa.gsi.gov.uk (fetlar.sasa.gsi.gov.uk [51.64.37.130]) by mail6.gsi.gov.uk (eggs/bacon) with ESMTP id g4MG7sL7017904 for <diagnost@net.bio.net>; Wed, 22 May 2002 17:07:54 +0100 (BST) X-Received: by sasa.gsi.gov.uk X-Received: by barra with Internet Mail Service (5.5.2653.19) id <JF9GDRX1>; Wed, 22 May 2002 17:07:52 +0100 X-To: "'diagnost@net.bio.net'" <diagnost@net.bio.net> X-Auth: PGPMoose V1.1 PGP bionet.diagnostics iQCVAwUAPOvDbqCIqsWvyF5FAQF7agP+LknT1706isGmjx5YpA3lUzo17QFmbp6u dGNPffipIDETUConfiDB3TKNLTVTABmZYWn2RUGjsKeiVrzIuXPDlpkIIYTdhpRG 8eb+UuCl0NIWz5dE2R4OtuuoCa2Brzcgi41CrL5JXGAKAF8T1xiwD2mDFXw5xwe6 n3F1VRVn9so= =9y9P