biotinylated protein for amplification

Article View: bionet.diagnostics

Article #126

Back to bionet.diagnostics Previous Next

#126

From: Tony O'Connor
Date: Wed, 05 Feb 2003 14:33

20 lines
1859 bytes

I want to biotinylate a protein to use as an "amplification layer" in
an ELISA, as follows:

[Solid-phase]-(Ab)-(Ag)-(biotinylated Ab)-(streptavidin)-
*{biotinylated protein}n*-(HRP-streptavidin)n

I'm doing this to replace a biotinylated anti-streptavidin Ab which is
not amplifying the signal. I'm going to try BSA - there's always BSA
in the lab :-)
Has anyone tried other proteins (adequately-sized with several
non-adjacent accessible amino groups) in such a system?

Thanks,
Tony

Tony O'Connor
Institute of Immunology
NUI Maynooth
Ireland
tony.oconnor@may.ie

Technical Details

Message-ID: <pgpmoose.200302051433.24714@net.bio.net>

Path:

arc1-ca.pugleaf.net!archiver1.google.com!news1.google.com!newsfeed.stanford.edu!bloom-beacon.mit.edu!hgmp.mrc.ac.uk!net.bio.net

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 10eaff,69f5609dda4df4d0,start
X-Google-Attributes: gid10eaff,public
X-Google-ArrivalTime: 2003-02-05 06:33:18 PST
Path: arc1-ca.pugleaf.net!archiver1.google.com!news1.google.com!newsfeed.stanford.edu!bloom-beacon.mit.edu!hgmp.mrc.ac.uk!net.bio.net
From: tony.oconnor@may.ie (Tony O'Connor)
Newsgroups: bionet.diagnostics
Subject: biotinylated protein for amplification
Date: 5 Feb 2003 14:33:16 -0000
Organization: http://groups.google.com/
Lines: 21
Sender: mod-diag@hgmp.mrc.ac.uk
Approved: BIOSCI Approval Key <biosci-approval-key@net.bio.net>
Message-ID: <pgpmoose.200302051433.24714@net.bio.net>
NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
X-Trace: niobium.hgmp.mrc.ac.uk 1044455597 3203 193.62.192.80 (5 Feb 2003 14:33:17 GMT)
X-Complaints-To: news@net.bio.net
NNTP-Posting-Date: Wed, 5 Feb 2003 14:33:17 +0000 (UTC)
X-Original-To: diagnost-submit@hgmp.mrc.ac.uk
X-Auth: PGPMoose V1.1 PGP bionet.diagnostics
 iQCVAwUAPkEgq6CIqsWvyF5FAQHUhwQAmr+K9RZZ7tmL3JeN+fm0WeI3MR8f0w4k
 Gf95fjrxEAUFgnXoJTblc+GzzUxlg6JPz1Q86iUITSf/XQx5GT/jx7Hpr6V7fo56
 7Kh18gcwoMUIqpr8DCwjo0fKNHv15pXTox+w9kwETPvKmytKCQeWpbVnFefxf8BM
 m2s1agHVJZc=
 =ALPk