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Article #1355Background staining in autoradiography.
From: David Getzy
Date: Thu, 22 Jun 1995 00:00
Date: Thu, 22 Jun 1995 00:00
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Folks, I have recently reviewed an autoradiography study for an experimental compound. The control animals of the group received saline only. In viewing the slides of the skin from the control animals, there is an increased deposition of silver grains between dermal collagen bundles. These spaces between collagen bundles are either processing artifacts or regions of cutaneous glycosaminoglycan accumulation. Any thoughts on the non-specific deposition of silver grains in this area of skin (as well as increased non-specific deposition on hair shafts)? Is is possible that the high GAG content of these tissues would bind silver cations through electrostatic forces? Thanks in advance for any help. DMG ********************************************************************* * Dave Getzy, Director * * Colo State Univ Diagnostic Laboratories * * 300 West Drake, Fort Collins, Co 80523 * * PHONE 970-491-1281 FAX 970-491-0320 * * EMAIL dgetzy@vth1.vth.colostate.edu * * http: //www.vth.colostate.edu * *********************************************************************
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,688dcec723c2913e,start X-Google-Attributes: gid10eaff,public From: dgetzy@VTH1.VTH.COLOSTATE.EDU (David Getzy) Subject: Background staining in autoradiography. Date: 1995/06/22 Message-ID: <Pine.A32.3.91.950622042135.27297E-100000@vth1.vth.colostate.edu> X-Deja-AN: 104939005 distribution: world sender: daemon@net.bio.net organization: BIOSCI International Newsgroups for Molecular Biology newsgroups: bionet.diagnostics