Re: "isolation" of haemoglobin

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#1402

From: Falk Fish
Date: Fri, 19 May 1995 00:00

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There should not be a problem resuspending hemoglobin from ammonium 
sulphate precipitate.  Just macerate the precipitate with a glass rod and 
water dropwise, while continuing to macerate.  At some point, the 
precipitate should dissolve into a clear solution.

You will now that the hemoglobin is not denatured if it is still red (the 
binding of the heme group to the protein requires an intact molecule).

If I am not mistaken, TCA will denature hemoglobin irreversibly.


Falk Fish, Tel Aviv, Israel.

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From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish)
Subject: Re: "isolation" of haemoglobin
Date: 1995/05/19
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