Re: Use of PVP in ELISA

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#1428

From: C. Liu
Date: Mon, 20 Mar 1995 14:45

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>As suggested by Bioreba we use polyvinylpyrrolidone in extraction and 
>conjugate buffers when carrying out ELISA for detection of virus in 
>plant sap. I would be grateful if someone could enlighten me as to why 
> it is used . Is its action as a blocker or as an antioxidant or does it have 
>some other function?
>
>
>Isla Browning
>SASA
>Edinburgh
>8/3/95
>
I believe it's used as a blocking agent in your assay.  It is a hydrophilic 
polymer that is a wetting agent, and probably reduces the nonspecific 
binding to plastic in microtiter plates.
---
cliu@ic.net
 
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From: cliu@IC.NET (C. Liu)
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Subject: Re: Use of PVP in ELISA
Date: 20 Mar 1995 06:45:35 -0800
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