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Article #1431Re: Use of PVP in ELISA
From: Prachya Kongtawelert
Date: Tue, 21 Mar 1995 01:16
Date: Tue, 21 Mar 1995 01:16
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As I have recalled, there was a paper concerning about the use of polyvinylpyrrolidone as a blocker in ELISA in Analytical Biochemistry, 1994. ************* Prachya Kongtawelert, Ph.D. Res Lab for Joint Dis., Dept of Biochem., Fac of Med., Chiangmai University, Chiangmai, Thailand 50200 Tel No. 66-53-894188 Fax No. 66-53-894188, 217144 email:mdbci001@chiangmai.ac.th On 20 Mar 1995, Isla Browning wrote: > As suggested by Bioreba we use polyvinylpyrrolidone in extraction and = > > conjugate buffers when carrying out ELISA for detection of virus in > plant sap. I would be grateful if someone could enlighten me as to why = > > it is used . Is its action as a blocker or as an antioxidant or does = > it have > some other function? > > > Isla Browning > SASA > Edinburgh > 8/3/95 > =01=01=01=01 > > > >
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,aaf8c1200d9e2008 X-Google-Attributes: gid10eaff,public X-Google-ArrivalTime: 1995-03-20 17:16:47 PST Path: arc1-ca.pugleaf.net!nntp.gmd.de!stern.fokus.gmd.de!ceres.fokus.gmd.de!zib-berlin.de!fu-berlin.de!news.dfn.de!swiss.ans.net!newsjunkie.ans.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!fnnews.fnal.gov!unixhub!news.Stanford.EDU!biosci!CMU.CHIANGMAI.AC.TH!mdbci001 From: mdbci001@CMU.CHIANGMAI.AC.TH (Prachya Kongtawelert) Newsgroups: bionet.diagnostics Subject: Re: Use of PVP in ELISA Date: 20 Mar 1995 17:16:47 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: <Pine.SUN.3.90.950321081407.5499A-100000@cmu.chiangmai.ac.th> References: <MAPI.Id.0016.00726f776e696e674432374130303341@MAPI.to.RFC822> NNTP-Posting-Host: net.bio.net