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Article #1433Re: Mabs from PAGE bands
From: Falk Fish
Date: Tue, 21 Mar 1995 04:20
Date: Tue, 21 Mar 1995 04:20
40 lines
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1528 bytes
On 16 Mar 1995, Robert Burns wrote: > Hi all, > my first posting to the "now permanent" diagnost list > > My question: Has anybody raised Mabs using protein bands excised > from PAGE gels as the immunogen? If so can you use the gel band > itself as an implant without having to elute the protein? > > Second question ( I have asked this before but for the benefit of > newcomers) Is anybody interested in Fungal diagnostics by serological > methods? > > > Robert > > > > > > Robert Burns > > Monoclonal Antibody Unit > Scottish Agricultural Science Agency > East Craigs > Edinburgh > Scotland > > burns@sasa.gov.uk > > > > Yes, you could immunize with the gel without elution, but macerate (grind) it first to smaller particles. Falk Fish.
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,8911cfa7a8769e86 X-Google-Attributes: gid10eaff,public X-Google-ArrivalTime: 1995-03-20 20:20:12 PST Path: arc1-ca.pugleaf.net!nntp.gmd.de!Germany.EU.net!EU.net!uunet!CS.Arizona.EDU!biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: Mabs from PAGE bands Date: 20 Mar 1995 20:20:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <Pine.3.89.1.2.9503210623.D365-0100000@zeus.datasrv.co.il> References: <MAPI.Id.0016.0075726e732020203636413830303030@MAPI.to.RFC822> NNTP-Posting-Host: net.bio.net