Re: low boiling point

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#1436

From: "Kevin O'Donnell"
Date: Tue, 21 Mar 1995 16:27

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<<Your message>>

 >We would like to initiate some PCR based methods near Quito,
 >Ecuador at an altitude of 3050 m above sea level.  Water boils
 >here at 89 C which will not allow us to bring the vials up above
 >this temperature.  Our PCR machine has not yet arrived but we
 >would like to have some ideas on how this problem might be solved
 >before it does.
 >Many thanks
 >Greg Forbes
<<End of your message>>

What an interesting problem! I somehow missed this when it was first posted.  
My immediate thought is: would this still be a problem if you had one of those 
thermal cyclers with a screw down heated lid?  Also, if water boils at a lower 
temperature, will the Tm of your DNA primers be lower as well?

Wish I knew more physics.    

Kevin O'Donnell		     
Diagnostics and Molecular Biology
Scottish Agricultural Science Agency    
Edinburgh                         		odonnell@sasa.gov.uk          
      

















Kevin O'Donnell		     
Diagnostics and Molecular Biology
Scottish Agricultural Science Agency    
Edinburgh                         		odonnell@sasa.gov.uk

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From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Re: low boiling point
Date: 21 Mar 1995 08:27:16 -0800
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