Use of PVP in ELISA

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From: "Collin, Roger"
Date: Tue, 21 Mar 1995 22:10

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With regard  to using PVP there is a paper in Analytical Biochemistry 
208:397-99, 1993 on "PVP as a blocking agent in immunochemical studies" byJW 
Haycock.
This paper recommends the use of PVP in conjunction with Tween 20 in Western 
Blots as it gives a high signal to background ratio.  I use this combination 
in an indirect competitive ELISA for sporidesmin (a mycotoxin) and have 
found that it works very well.

Regards Dr Roger Collin
AgResearch, Hamilton, New Zealand

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From: COLLINR@agresearch.cri.nz ("Collin, Roger")
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Subject: Use of PVP in ELISA
Date: 21 Mar 1995 14:10:29 -0800
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