RE:low boiling point

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#1444

From: SMASOUD
Date: Thu, 23 Mar 1995 17:20

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In addtion of increasing the boiling temp., DMSO weaken the douple strand
interactions.  This should lower the annealing temp. and lower denaturing temp.
I would recomend playing with both parammeters if you want to use DMSO in PCR.
I have used DMSO before in PCR and was very effective.  I found that
you need to find the optimum concentration for any DNA/primers combination.
DMSO inhibit taq activity at high concentrations.

Sameer Masoud
Plant Biology Division
S. R. Noble Foundation
2510 Sam Noble Parkway
Ardmore, OK 73402
Tel (405) 221 7309
FAX (405) 221 7380

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From: SMASOUD@AARDVARK.UCS.UOKNOR.EDU
Newsgroups: bionet.diagnostics
Subject: RE:low boiling point
Date: 23 Mar 1995 09:20:11 -0800
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