Article View: bionet.diagnostics
Article #1449PCR at high altitude
From: David Watson
Date: Thu, 23 Mar 1995 22:36
Date: Thu, 23 Mar 1995 22:36
17 lines
1543 bytes
1543 bytes
Dear Netters:
A quick observation (somebody correct me if this is not accurate):
Taq is an enzyme, albeit one with a very high temperature optimum.
Therefore, at a temp slightly below boiling in Quito (89 C, was it?),
shouldn't Taq still work, even if slightly less than optimally, such that
routine amplifications could still be accomplished? Does anyone know how
rapidly Taq's efficiency falls off either side of 94 C? I assume the dropoff
is steeper above 94 C?
|===========================================|
David A. Watson, Ph.D. (dwatson@plains.nodak.edu)
Asst. Prof. of Veterinary and Microbiological Sciences
North Dakota State University
---------------------------------------------------------------------------
"Some are wise, some otherwise."
|===========================================|
Technical Details
Message-ID:
<199503232235.AA11783@plains.NoDak.edu>Path:
arc1-ca.pugleaf.net!nntp.gmd.de!Germany.EU.net!EU.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!biosci!PLAINS.NODAK.EDU!dwatsonShow Full Headers
Complete Article Headers
X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,986a0573cd4a4cb0,start X-Google-Attributes: gid10eaff,public X-Google-ArrivalTime: 1995-03-23 14:36:08 PST Path: arc1-ca.pugleaf.net!nntp.gmd.de!Germany.EU.net!EU.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!biosci!PLAINS.NODAK.EDU!dwatson From: dwatson@PLAINS.NODAK.EDU (David Watson) Newsgroups: bionet.diagnostics Subject: PCR at high altitude Date: 23 Mar 1995 14:36:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199503232235.AA11783@plains.NoDak.edu> NNTP-Posting-Host: net.bio.net