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Article #1481Re: Morphine and ELISA
From: Falk Fish
Date: Tue, 18 Apr 1995 00:00
Date: Tue, 18 Apr 1995 00:00
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On 13 Apr 1995, Frederic Bourgaud wrote: > Hi all, > > I am starting a new research program dealing with morphine in plants. I am > looking for a rapid/reliable ELISA test that would be adapted to this. I > already know that ELISA tech.(Lexington, Kentucky) commercialises a kit > called "generic opiate ELISA test". It's a competition test and involves > polyclonal antobodies. It is not very specific to morphine as it crosses > with all the related alkaloids. It costs about 90 US $ per plate. > > Does anybody know another ELISA test, maybe based on monoclonal antibodies > ? It is important to me to have a test more specific to morphine. Also any > experience/reference on morphine quantification in plants with ELISA would > be appreciated. > > Also posted to the Biotech and Plant-TC discussion lists. Sorry for > multiple postings. > > Thanks > > Fred (bourgaud@ensaia.u-nancy.fr) For tests for morphine and other drugs of abuse, you can try the following: Hanson Hog Biomedical Co., Taipei, Taiwan, Tel +886-2-7922808, Fax ..7914624 Immunotech Corp, Boston, MA, Tel +1-617-787-1010, Fax ..787-0315 Princeton Biomeditech, Princeton, NJ, Tel +1-908-274-1000, Fax ..274-1010 BiosPacific, Emeryville, CA, Tel +1-415-652-6155, Fax ..652-4531 Eucardio Laboratory, San Diego, CA, Tel +1-619-689-8719, Fax ..689-8719 (The above addresses are from my own database, so some or all may be outdated). As to the specificity of the assay, I think that in your case, less specificity is preferable for initial screening. Once you find a plant with traces of your material, try to determine the exact molecule. Falk Fish, Tel-Aviv, Israel.
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: 10eaff,a5ce73dfc4091be6,start X-Google-Attributes: gid10eaff,public From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Subject: Re: Morphine and ELISA Date: 1995/04/18 Message-ID: <Pine.3.89.1.2.9504190129.A26321-0100000@zeus.datasrv.co.il> X-Deja-AN: 101137634 distribution: world sender: daemon@net.bio.net references: <3min8b$4o5@mserv1.dl.ac.uk> organization: BIOSCI International Newsgroups for Molecular Biology newsgroups: bionet.diagnostics