identification of rDNA

Article View: bionet.diagnostics

Article #190

Back to bionet.diagnostics Previous Next

#190

From: nobody mailtonobody On Behalf Of
Date: Thu, 16 Nov 2000 00:00

15 lines
1125 bytes

Dear colleagues,
I want to check plasmid inserts in a Southern HYB for clones with 
repetitive sequences. So I am planning to hybridyze with maize 
genomic DNA to identify these rDNA containing clones. I am not 
sure about the amount of labelled probe to use for this HYB. For a 
normal Southern with a certain fragment as probe i use 50-100ng 
(P32-labelling). But is this enough to cover all species of rDNA 
within genomic DNA?
Thanks for your answers in advance,

Marcel


---

Technical Details

Message-ID: <8v0kjo$gqm$1@mercury.hgmp.mrc.ac.uk>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 10eaff,d256ab291108c611,start
X-Google-Attributes: gid10eaff,public
From: nobody@hgmp.mrc.ac.uk [mailto:nobody@hgmp.mrc.ac.uk] On Behalf Of
Subject: identification of rDNA
Date: 2000/11/16
Message-ID: <8v0kjo$gqm$1@mercury.hgmp.mrc.ac.uk>
X-Deja-AN: 694301619
Approved: burns@sasa.gov.uk
X-Trace: niobium.hgmp.mrc.ac.uk 974378426 23830 193.62.192.80 (16 Nov 2000 12:40:26 GMT)
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
NNTP-Posting-Date: 16 Nov 2000 12:40:26 GMT
Newsgroups: bionet.diagnostics
X-Complaints-To: news@net.bio.net