Microbial DNA isolation from WBC with Chelex 100?

Article View: bionet.diagnostics

Article #53

Back to bionet.diagnostics Previous Next

#53

From: Mikhail Edelstein mailtome
Date: Wed, 27 Oct 1999 00:00

11 lines
909 bytes

I am looking for a method of microbial DNA extraction from white cell
fraction of blood using the Chelex 100 resine.
I am particularly interested in isolation of C.pneumoniae DNA with
Chelex 100 for subsequent detection by PCR. However, I would appreciate
any reply from those who use Chelex for processing PCR blood samples.

Sincerely,

Michael Edelstein.
mailto: me@cliph.keytown.com

Technical Details

Message-ID: <7v74je$onl@net.bio.net>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 10eaff,d48f7688cb3b99fc,start
X-Google-Attributes: gid10eaff,public
From: Mikhail Edelstein [mailto:me@casus.cliph.keytown.com]
Subject: Microbial DNA isolation from WBC with Chelex 100?
Date: 1999/10/27
Message-ID: <7v74je$onl@net.bio.net>
X-Deja-AN: 541229564
Distribution: world
Approved: odonnell@sasa.gov.uk
Sender: daemon@net.bio.net
Organization: BIOSCI International Newsgroups for Molecular Biology
Newsgroups: bionet.diagnostics