detection of viable moulds on RNA basis

Article View: bionet.diagnostics

Article #79

Back to bionet.diagnostics Previous Next

#79

From: daemon mailtodaemon On Behalf Of
Date: Wed, 09 Feb 2000 00:00

11 lines
1051 bytes

Does anybody know the half life time of free ribosomal DNA in a 
neutral solution?
My idea is to detect in a quantitive way the amount of viable 
moulds on basis of RNA. The idea is that RNA has a short half life 
and therefor a RT PCR of freshly isolated total RNA could tell 
something about the number of viable cells. However I am not sure 
about the stability of ribosomal RNA.

Edwin
---

Technical Details

Message-ID: <87rnpb$bkg$1@mercury.hgmp.mrc.ac.uk>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 10eaff,6e8b442f02f1e6c2,start
X-Google-Attributes: gid10eaff,public
From: daemon@hgmp.mrc.ac.uk [mailto:daemon@hgmp.mrc.ac.uk] On Behalf Of
Subject: detection of viable moulds on RNA basis
Date: 2000/02/09
Message-ID: <87rnpb$bkg$1@mercury.hgmp.mrc.ac.uk>
X-Deja-AN: 583635961
Approved: odonnell@sasa.gov.uk
X-Trace: niobium.hgmp.mrc.ac.uk 950100587 1736 193.62.192.80 (9 Feb 2000 12:49:47 GMT)
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
NNTP-Posting-Date: 9 Feb 2000 12:49:47 GMT
Newsgroups: bionet.diagnostics
X-Complaints-To: news@net.bio.net