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Article #86dgge
From: daemon mailtodaemon On Behalf Of
Date: Tue, 22 Feb 2000 00:00
Date: Tue, 22 Feb 2000 00:00
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915 bytes
i make amplification with primers that give a 700bp fragment. i had tried to make migration in 25 to 35% d�naturant gel at 75�C but for three time i had obtained no s�paration and the last time there was no band. can you explain me this or can you show me a reference for this thank you ---
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X-Google-Language: ENGLISH,ASCII X-Google-Thread: 10eaff,3b5abdd6fa9b1cbc,start X-Google-Attributes: gid10eaff,public From: daemon@hgmp.mrc.ac.uk [mailto:daemon@hgmp.mrc.ac.uk] On Behalf Of Subject: dgge Date: 2000/02/22 Message-ID: <88u8fn$q9v$1@mercury.hgmp.mrc.ac.uk> X-Deja-AN: 588414280 Approved: odonnell@sasa.gov.uk X-Trace: niobium.hgmp.mrc.ac.uk 951231799 13245 193.62.192.80 (22 Feb 2000 15:03:19 GMT) Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre NNTP-Posting-Date: 22 Feb 2000 15:03:19 GMT Newsgroups: bionet.diagnostics X-Complaints-To: news@net.bio.net