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Article #2095Re: DNA and RNA extraction
From: Patrick
Date: Sun, 02 Jun 1996 00:00
Date: Sun, 02 Jun 1996 00:00
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1396 bytes
On Thu, 30 May 1996 mazzocco@sirio.cba.unige.it wrote: > Hi, is there anyone who could help me? > Is it possible doing a simultaneously DNA and RNA extraction from frozen bone > marrow, without using cesium cloride? Certainly. We quit using the cesium chloride method late last year (it would have been sooner if we had gotten our way but our PI tends to be somewhat conservative about some things). Not to push a particular company but Qiagen makes nice mini and maxi columns for very clean nucleic acid purification. The DNA is clean enough for microinjection into mouse embryos for creating transgenics...the prime test (and concern) for our lab. There are other such products from other companies as well. patrick
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: f8e22,90db71367fb91f12 X-Google-Attributes: gidf8e22,public X-Google-Thread: 1119a6,90db71367fb91f12 X-Google-Attributes: gid1119a6,public From: Patrick <patrick@howard.genetics.utah.edu> Subject: Re: DNA and RNA extraction Date: 1996/06/02 Message-ID: <Pine.SOL.3.91.960602231407.8500C-100000@howard> X-Deja-AN: 158177059 references: <NEWTNews.833499105.7296.mazzocco@cassiopea.cba.unige.it> x-sender: patrick@howard content-type: TEXT/PLAIN; charset=US-ASCII organization: University of Utah Computer Center mime-version: 1.0 newsgroups: bionet.general,bionet.cellbiol