Jurkat transient transfection protocol

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Article #2219

#2219

From: Andrew Suh
Date: Fri, 03 Sep 2010 08:25

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Hi everyone,

I am trying to transfect some Jurkat cells using the DEAE-dextran method.

I am only getting abou5 2-5% transfection efficiency. I was wondering if anyone can send me a protocol that gives a lot better transfection efficiency for this cell line. Please either reply to this forum of email me neoret at msn dot com. 

Thanks in advance for your help.

David

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