Re: Tricine gels

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Article #2225

#2225

From: Dr Engelbert Buxbaum
Date: Thu, 30 Sep 2010 22:50

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In article <b11e3d17-d3f6-40b8-9e44-
e1fca883fc0e@q40g2000prg.googlegroups.com>, aannamma@gmail.com says...
> 
> We have been trying to do tricine gels and have been running the gels
> at 90V... However we have observed extensive heating up of the gel
> assembly.

It is usually more effective to run gels at constant current, 10-20 mA 
per small (8x6 cm^2) gel. You may also use a cooled system, e.g., 
Hoefer. 

Other than that, keep conductivity as low as possible. Some heating is, 
however, inevitable. If you run a gel at 20 mA, the voltage will 
increase during the run from 60 to 200 V, that's up to 4 W powered into 
some 2 ml gel volume for 1 h or so.

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From: Dr Engelbert Buxbaum <engelbert_buxbaum@hotmail.com>
Newsgroups: bionet.general
Subject: Re: Tricine gels
Date: Thu, 30 Sep 2010 18:50:54 -0400
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