Palindrome and non-palindrome

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#491

From: Ana Maldonado
Date: Tue, 23 Jun 2009 11:43

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Dear Dr. Gegenheimer,
I hope this e-mail finds you well.

I sought that you gave some reasons to explain why palindromic recognition
sequences.
I found that you answer is very reasonable and indeed, because the risk or
having this flanks the bacteria avoid those sequences. However, what about
non-palindromic restriction sites? Those are avoided too, but are those as
important as palindromic sequences? The non-symmetric restriction sites will
be target of a single-stranded cleavage or same as palindrome, the enzymes
can cut both DNA strands?

I will really appreciate your input.

Ana Luisa

-- 
Ana Luisa Maldonado-Contreras
PhD Candidate
University of Puerto Rico
Rio Piedras Campus
Microbial Ecology Lab
Phone: 787 764 0000 ext. 4883

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<span class=3D"Apple-style-span" style=3D"font-family: Helvetica; font-size=
: 12px; "><div style=3D"word-wrap: break-word; -webkit-nbsp-mode: space; -w=
ebkit-line-break: after-white-space; ">Dear Dr. Gegenheimer,<br>I hope this=
 e-mail finds you well.<br>
<br>I sought that you gave some reasons to explain why palindromic recognit=
ion sequences.<br>I found that you answer is very reasonable and indeed, be=
cause the risk or having this flanks the bacteria avoid those sequences. Ho=
wever, what about non-palindromic restriction sites? Those are avoided too,=
 but are those as important as palindromic sequences? The non-symmetric res=
triction sites will be target of a single-stranded cleavage or same as pali=
ndrome, the enzymes can cut both DNA strands?<br>
<br>I will really appreciate your input.<br><br>Ana Luisa</div></span><br>-=
- <br>Ana Luisa Maldonado-Contreras<br>PhD Candidate<br>University of Puert=
o Rico<br>Rio Piedras Campus<br>Microbial Ecology Lab<br>Phone: 787 764 000=
0 ext. 4883<br>


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From: Ana Maldonado <almaldon@gmail.com>
Newsgroups: bionet.molbio.evolution
Subject: Palindrome and non-palindrome
Date: Tue, 23 Jun 2009 07:43:52 -0400
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