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TECHNOLOGIES FOR GENERATION OF FULL-LENGTH MAMMALIAN cDNA

Release Date:  March 5, 1999
RFA NUMBER:  RFA-CA-99-005
Letter of Intent Receipt Date:  April 6, 1999
Application Receipt Date:  May 13, 1999

In an effort to provide the research community with high quality, full-length
mammalian cDNA clones and sequences, the National Institutes of Health (NIH) 
has
established the Full-Length cDNA Initiative, managed by a trans-NIH steering
committee.  Ultimately, the clones and sequences produced through this
initiative will provide a "gold standard" set of reagents for use by the
research community.  This Request for Application (RFA) targets one 
component of
the initiative: cDNA cloning technology development.

The purpose of this RFA is to support the development of technologies that will
facilitate the generation of a complete set of full-length human cDNAs as well
as other mammalian cDNAs.  Current methods of cDNA clone and library production
favor shorter, more heavily represented genes.  In addition, although current
methodology for isolating mRNA for use in cDNA construction works well with 
cell
lines, reliable methodologies for extraction of high quality mRNA from tissues
remains a challenge.  Use of human tissues may be necessary to achieve the goal
of a complete set of human cDNA clones. Finally, reliable, high-throughput
methods to determine whether clones contain a copy of the full transcript, the
full coding region, or a partial transcript are needed.  This RFA is 
intended to
support innovative research projects aimed at solving one or more of the
problems currently associated with the production of a complete set of
full-length human cDNA clones and full-length cDNA clones from other mammals.

Additional information about the RFA can be found at:
http://www.nih.gov/grants/guide/rfa-files/RFA-CA-99-005.html