southern rehybridisation problem

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1 total messages Started by Edith Duerbaum Thu, 21 Jan 1999 00:00

#436

Author: Edith Duerbaum
Date: Thu, 21 Jan 1999 00:00

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Dear All,

I have a problem:

Today I rehybridised three older membranes (6 months-1 year) that I had
stored in paper (the nice sheets that come with Kodak films) but with no
other precautions. The cupboard contains no chemicals except
mercaptoethanol-solution, just glassware.
Two membranes appear to be free of DNA, one gave very faint signals.

What can be the cause? Is it the storage at ambient humidity etc, the
mercaptoethanol-solution or ....? All the membranes worked fine when I
hybridised and rehybridised them first ....

Please give me our ideas.

Thanks,

  Edith

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