Extraction Of Proteins From Zebrafish Embryos

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2 total messages Started by cl_shi Wed, 29 Feb 2012 03:52

Author: cl_shi
Date: Wed, 29 Feb 2012 03:52

6 lines
2778 bytes

Just wondering whether you still extract your proteins following the
ZF protocol with ''Immerse tube in boiling water for 1 minute''??
Since someone has report it may cause to form very HMW band after
heating.

CS

Re: Extraction Of Proteins From Zebrafish Embryos

#48

Author: 姜郁琦
Date: Tue, 30 Oct 2012 01:14

18 lines
2991 bytes

Maybe your sample buffer didn't contain enough beta-ME or DTT, so that It formed  polymer .


chi


cs於 2012年2月29日星期三UTC+8上午11時52分07秒寫道：
> Just wondering whether you still extract your proteins following the
> 
> ZF protocol with ''Immerse tube in boiling water for 1 minute''??
> 
> Since someone has report it may cause to form very HMW band after
> 
> heating.
> 
> 
> 
> CS

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