Thread View: bionet.organisms.zebrafish
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Started by Peggy Jungke
Thu, 31 May 2012 08:20
CreERT2 driver lines-database
Author: Peggy Jungke
Date: Thu, 31 May 2012 08:20
Date: Thu, 31 May 2012 08:20
32 lines
3669 bytes
3669 bytes
Dear zebrafish colleagues, In the past years the Cre/lox system has been established in zebrafish to complement the existing reverse genetic applications like Gal4/UAS or mifepristone-inducible LexPR. Cre-mediated recombination has been shown by several labs to be highly efficient in the developing and adult zebrafish and temporal control of recombination is achieved by using the ligand-inducible CreERT2. To broaden the pool of existing CreERT2 driver lines, we recently conducted a gene trap screen and established more than 40 transgenic lines that express CreERT2 in various tissues. To share and distribute this data within the zebrafish community, we composed an easy-to-handle database (http://crezoo.crt-dresden.de). This database contains the embryonic expression profiles, but also additional information (e.g. mCherry or CreERT2 expression at adult stages, transactivation of a Cre-dependent reporter line). Currently, not all insertions have been mapped to a genomic location but the database is regularly updated, adding newly generated insertions and mapping information. For further information, please visit http://crezoo.crt-dresden.de. Best wishes, Michael Brand Stefan Hans Peggy Jungke Center for Regenerative Therapies, CRTD TU Dresden Germany http://www.crt-dresden.de
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