Protocol for BB:BA mounting

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2 total messages Started by Nagganatha Suganthan R Sat, 29 Sep 2012 05:22

#41

Author: Nagganatha Suganthan R
Date: Sat, 29 Sep 2012 05:22

11 lines
2791 bytes

Dear friends

I humbly request anyone in our group to kindly tell me the protocol
for mounting the stained embryos in BB:BA mixture starting from the 4%
PFA fixation.

Thanks and Regards
-- 
R Nagganatha Suganthan
Research Scholar
Ehime University

Re: Protocol for BB:BA mounting

#64

Author: vkorzh
Date: Fri, 28 Dec 2012 09:26

28 lines
3252 bytes

Hi,
Since I worked long ago with this protocol, I do not recommend you to use it. It is smelly and toxic, so ideally you should work all the time under fume hood but try to work with your microscopes there. You will be much better off by mounting embryos into 50% glycerol.
Sometime it is easier to repeat staining and not to use BB:BA destaining.
Best,
VK

On Saturday, September 29, 2012 1:22:07 PM UTC+8, Harry wrote:
> Dear friends
> 
> 
> 
> I humbly request anyone in our group to kindly tell me the protocol
> 
> for mounting the stained embryos in BB:BA mixture starting from the 4%
> 
> PFA fixation.
> 
> 
> 
> Thanks and Regards
> 
> -- 
> 
> R Nagganatha Suganthan
> 
> Research Scholar
> 
> Ehime University

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