Using oligo probes for zebrafish WMISH

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1 total messages Started by Nagganatha Suganthan R Tue, 02 Oct 2012 01:48

#42

Author: Nagganatha Suganthan R
Date: Tue, 02 Oct 2012 01:48

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Almost all the zebrafish WMISH protocols say about preparing and using
the riboprobes for RNA hybridization. Several ISH protocols for tissue
sections allow oligo probes (20-40bp) to be used for detecting target
mRNA . So can they also be used for zebrafish embryos also with right
hybridisation conditions?

-- 
R Nagganatha Suganthan
Research Scholar
Ehime University
Japan

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