Cell Viability Assay or Antibody

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1 total messages Started by greengirl1808 Tue, 06 Nov 2012 01:04

#49

Author: greengirl1808
Date: Tue, 06 Nov 2012 01:04

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I am currently using Acridine orange to analyze cell death/apoptosis in zebrafish embryos. My committee asked me to show/prove that the other cells are actually alive. Does anyone know a good method to show cell viability in ZF embryos? Possibly a vital dye or antibody (essential in cells that are still alive but not present in apoptotic cells) to use in immunhistochemitry?

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