Thread View: bionet.organisms.zebrafish
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Started by Keith Joung
Sun, 18 Nov 2012 13:07
Re: THIRD Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases
Author: Keith Joung
Date: Sun, 18 Nov 2012 13:07
Date: Sun, 18 Nov 2012 13:07
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Dear colleagues, We write to provide an update on the status of our THIRD solicitation for constructing engineered nucleases to target zebrafish genes. As you may recall, for this round we are seeking to produce TALEN pairs for 96 different target genes. We are pleased to report that we have deposited plasmids encoding TALEN pairs with Addgene for 76 of these 96 genes (see list below). We hope to deposit the TALEN pairs for the remaining 20 target genes (also listed below) in the next few weeks and will post a message when this occurs. After Addgene has completed their sequence-verification process in the coming weeks, these new TALEN plasmids will be available here: http://www.addgene.org/talengineering/TALENzebrafish/. If you are interested in requesting one of these new plasmids, please contact Jason Niehaus at Addgene (jason@addgene.org) and he can let you know when your specific plasmids of interest become available. We plan to announce a new FOURTH solicitation in the next few days. The announcement will be posted to this newsgroup. With best regards, Keith Joung Randy Peterson Joanna Yeh Jonathan Foley Deepak Reyon Jeffry Sander 76 zebrafish genes for which TALENs have been deposited with Addgene: adam15 apoa4 bbc3-(puma) bmp10 bsx btr30 casp8ap2 cebpa cebpd cish.b-(socs8) cited4a cited4b clcn1a cln3 cx52.6 ednrb1b egf-like-domain eml2 fan1 fascin-2b gabra4 gabrg2 gata2b gbas ghsra ghsrb gna13a gna13b gopc her6 hnrnpk hsd17b4 id2a ippk irf6 kinesin-family-member-13a kiss2 lyl1 mcu mdm2 meis1 meis3 mir-726 musashi-2a mycn nphs2 nptx2a olig3 parn patz1 per2 plekho1b-001 pum1 pycr1 rbfox1l ryr2b scoc-b slc26a6 smad2 smad3b smad9 snai2 socs3b solute-carrier-family-16 sox2 spry4 stac svila tac3a tes tfeb tfec ulk2 vascular-endothelia-growth-factor-aa-(vegfaa) zbtb7b znf385c 20 zebrafish genes for which TALENs will be deposited with Addgene in the coming weeks: ahcyl1 aminopeptidase-o apoa4-like arap3a avpr1b clcn1b cx52.7 dazl elk1 ets1a nr2e3 pex2 rasa1a rasa1b rasd1-201 rps19 scoc-a sox9b tmem88 zgc-193690
Re: THIRD Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases
Author: Keith Joung
Date: Fri, 14 Dec 2012 01:09
Date: Fri, 14 Dec 2012 01:09
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A quick update to let you know that all of the 96 pairs of TALENs made for our THIRD NIH-sponsored solicitation to construct nucleases for zebrafish genes have now been deposited with Addgene. A complete list of these plasmids (as well as additional TALENs made previously) can be found here: http://www.addgene.org/talengineering/TALENzebrafish/. At present, approximately eighty of the 96 pairs are available with the remainder coming in the next few weeks. If you would like to receive notice of when a plasmid becomes available, please contact Jason Niehaus (cc:d) at Addgene. With best regards, Keith Joung Randy Peterson Joanna Yeh Jonathan Foley Deepak Reyon Jeffry Sander
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