protocol for western blot for phopho-proteins on very early embryos (pre-MBT)

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1 total messages Started by xiang.zhangm Wed, 12 Dec 2012 21:27

#61

Author: xiang.zhangm
Date: Wed, 12 Dec 2012 21:27

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Hi all,

I am starting to do some westerns for phospho-proteins for Chk1 and Chk2. I am working with very early embryos (as early as 2 hpf to around 6 hpf). I've been having very little success with my antibodies (usually, I'll have no bands).  Does anyone have a protocol they've used with success?

Thanks!

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