Thread View: bionet.organisms.zebrafish
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Started by Burdine, Rebecca D.
Tue, 15 Jan 2013 18:11
RNA injection question
Author: Burdine, Rebecca D.
Date: Tue, 15 Jan 2013 18:11
Date: Tue, 15 Jan 2013 18:11
165 lines
10217 bytes
10217 bytes
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Hi everyone,
We have routinely used the mMessage mMachine kits to make RNA for injection=
s experiments, both for overexpression and for rescue.
We have never used the polyA tailing kit available from this company. I am=
wondering if that is an oversight.
Does anyone have a feeling for whether or not we should be routinely using =
the polyA kit when generating RNA? Has anyone done a comparison to see if =
it makes a big difference? Do people pay attention to whether or not their=
rescue construct contain sequences for in vivo poly A addition?
Any insight would be appreciated.
Becky
---------------------------------------------------
Rebecca D. Burdine, Ph.D.
Assistant Professor
Dept. of Molecular Biology
Princeton University
Washington Road Mof 433
Princeton, NJ 08544
Phone: (609) 258-7515
Fax: (609) 258-6730
Email: rburdine@princeton.edu<mailto:rburdine@princeton.edu>
Admin Assistant: Anna Schmedel (609) 258-5028
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<p class=3D"MsoNormal">Hi everyone,<o:p></o:p></p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
<p class=3D"MsoNormal">We have routinely used the mMessage mMachine kits to=
make RNA for injections experiments, both for overexpression and for rescu=
e.<o:p></o:p></p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
<p class=3D"MsoNormal">We have never used the polyA tailing kit available f=
rom this company. I am wondering if that is an oversight.<o:p></o:p><=
/p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
<p class=3D"MsoNormal">Does anyone have a feeling for whether or not we sho=
uld be routinely using the polyA kit when generating RNA? Has anyone =
done a comparison to see if it makes a big difference? Do people pay =
attention to whether or not their rescue construct
contain sequences for in vivo poly A addition?<o:p></o:p></p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
<p class=3D"MsoNormal">Any insight would be appreciated. <o:p><=
/o:p></p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
<p class=3D"MsoNormal">Becky <o:p></o:p></p>
<p class=3D"MsoNormal" style=3D"margin-left:.5in"><o:p> </o:p></p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">-----------------------------=
----------------------</span><span style=3D"font-family:"Arial",&=
quot;sans-serif";color:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Rebecca D. Burdine, Ph.D.</sp=
an><span style=3D"font-family:"Arial","sans-serif";colo=
r:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Assistant Professor</span><sp=
an style=3D"font-family:"Arial","sans-serif";color:#1F4=
97D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Dept. of Molecular Biology</s=
pan><span style=3D"font-family:"Arial","sans-serif";col=
or:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Princeton University</span><s=
pan style=3D"font-family:"Arial","sans-serif";color:#1F=
497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Washington Road Mof 433</span=
><span style=3D"font-family:"Arial","sans-serif";color:=
#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Princeton, NJ 08544 </sp=
an><span style=3D"font-family:"Arial","sans-serif";colo=
r:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-family:"Arial","s=
ans-serif";color:black"> </span><span style=3D"font-family:"=
Arial","sans-serif";color:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Phone: (609) 258-7515</s=
pan><span style=3D"font-family:"Arial","sans-serif";col=
or:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Fax: (609) 258-6730</span><sp=
an style=3D"font-family:"Arial","sans-serif";color:#1F4=
97D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Email:
<a href=3D"mailto:rburdine@princeton.edu" target=3D"_blank"><span style=3D"=
color:blue">rburdine@princeton.edu</span></a></span><span style=3D"font-fam=
ily:"Arial","sans-serif";color:#1F497D"><o:p></o:p></sp=
an></p>
<p class=3D"MsoNormal"><span style=3D"font-size:10.0pt;font-family:"Ar=
ial","sans-serif";color:black">Admin Assistant: Anna Schmede=
l (609) 258-5028</span><span style=3D"font-family:"Arial","s=
ans-serif";color:#1F497D"><o:p></o:p></span></p>
<p class=3D"MsoNormal"><o:p> </o:p></p>
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