Thread View: bionet.organisms.zebrafish
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Started by Keith Joung
Sun, 10 Feb 2013 00:28
Announcement of FIFTH (and FINAL) Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases
Author: Keith Joung
Date: Sun, 10 Feb 2013 00:28
Date: Sun, 10 Feb 2013 00:28
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5338 bytes
Dear Colleagues, We are writing again to solicit recommendations for appropriate genes to target in our FIFTH (and FINAL) solicitation of zebrafish genes to target with engineered nucleases. As in the previous (fourth) round, we plan to construct 96 TALEN pairs. (Note that plasmids for the 96 pairs made for the fourth solicitation are nearly completed and will be deposited with Addgene by the end of February.) Each laboratory may submit up to TWO gene targets by completing an Excel form that can be downloaded here: http://www.talengineering.org/download.htm. Note that one gene recommendation should be placed on each of the "Sheets" in the file. Place your first choice gene on the first sheet and your second choice gene on the second sheet. Please note that ALL required fields must be filled out - we will not accept or consider any incomplete forms. For a gene to be considered appropriate for this effort, we ask each recommender to verify on the form that mutations in the gene of interest do not already exist and that efforts to generate mutations in the recommended gene are not underway elsewhere. VERY IMPORTANT NOTE: Each gene submitted MUST include its Ensembl Gene ID (ENSDARG) the Ensembl Transcript ID (ENSDART), and the gene name (e.g.-- Example: ENSDARG00000024771, ENSDART00000033574, slc24a5). In the event that Ensembl has incorrectly annotated the gene of interest, the full genomic sequence of your gene should be included with exons denoted by upper case and introns in lower case. Genes not correctly submitted in one of these formats will NOT be considered for targeting. Recommendations will be considered in the order in which they are received. We will choose one gene for targeting from each lab until we reach 96 gene targets. If we receive fewer than 96 targetable "first choice" gene requests, we will then consider "second choice" requests in the order in which we received the e-mails until we reach 96 gene targets. Please e-mail your completed form to zebrafishzfs@zincfingers.org at or after (and not before) 12:00 Noon (Eastern US time) Friday, February 22, 2013. Requests received before this date/time will not be considered. Gene recommendations will only be accepted via e- mail. Note that recommendations from scientists at commercial entities will not be accepted. With best regards, Keith Joung Randall Peterson Joanna Yeh Jeffry Sander Deepak Reyon Jon Foley Massachusetts General Hospital
Re: Announcement of FIFTH (and FINAL) Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases
Author: Keith Joung
Date: Fri, 01 Mar 2013 02:51
Date: Fri, 01 Mar 2013 02:51
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Dear all, We are pleased to announce the list of 96 zebrafish genes and target sites for which we will make FLASH-assembled TALENs for the community as part of our FIFTH (and FINAL) NIH-sponsored solicitation can be found below. We hope to deposit the plasmids encoding these TALENs with Addgene in the coming months. We will write with updates as we make those deposits. Best, Keith Joung Randall Peterson Joanna Yeh Jon Foley Deepak Reyon Jeff Sander Massachusetts General Hospital ID Gene Name Target Site 1 ZebrafishCommunity-abcg2a-left TGTCCTCATCCCCGTCCC 1 ZebrafishCommunity-abcg2a-right TTGATGTTGTGGAAGCTG 2 ZebrafishCommunity-apoeb-left TGCCAGGCTCGTAGCCTG 2 ZebrafishCommunity-apoeb-right TCCTCCCATCTGGGCTGA 3 ZebrafishCommunity-asun-left TAACTCAAGCCAACTCCA 3 ZebrafishCommunity-asun-right TGAACTCAATCTTCCTGC 4 ZebrafishCommunity-atf6-left TCCAACCAGCAAAAAGAC 4 ZebrafishCommunity-atf6-right TAGGCTGAATGGAAACCT 5 ZebrafishCommunity-CHCHD4-left TCTACCTAATGGCGACAT 5 ZebrafishCommunity-CHCHD4-right TGGCCATGCCGCCCAAAC 6 ZebrafishCommunity-col22A1-left TGGATGGCGAGAACAGGG 6 ZebrafishCommunity-col22A1-right TCCTTCGTCAGGCCCACA 7 ZebrafishCommunity-Cyp19a1-left TGCTCCAGCCCTGTGGAA 7 ZebrafishCommunity-Cyp19a1-right TCCACCACAGCCTCGCCG 8 ZebrafishCommunity-CYP20A1-left TAGACTTTCCTGAAAAGC 8 ZebrafishCommunity-CYP20A1-right TGTCACAGCAAAAATGGC 9 ZebrafishCommunity-dcps-left TGAACAGTCCACCGATAA 9 ZebrafishCommunity-dcps-right TAGAATGCGGGTGAGCTG 10 ZebrafishCommunity-ddx26b-left TGAAGGCCAGCACGACAC 10 ZebrafishCommunity-ddx26b-right TTGTAAGGCATGACGAAG 11 ZebrafishCommunity-def6a-left TCAGAACTGCTCAAGTCC 11 ZebrafishCommunity-def6a-right TCTCCACATCCAGAGCTG 12 ZebrafishCommunity-DLK1(different_cut)-left TGAGGTACAGGCAGCTGG 12 ZebrafishCommunity-DLK1(different_cut)-right TGGCCTGGTCCTGGATCA 13 ZebrafishCommunity-dyrk1aa-left TGTTCCGAAACCACCTGT 13 ZebrafishCommunity-dyrk1aa-right TACAGGTTATAGGACAGC 14 ZebrafishCommunity-dyrk1b-left TCCTGAACCAGGCCCAGA 14 ZebrafishCommunity-dyrk1b-right TGTTTGTTCATGAGCTCA 15 ZebrafishCommunity-eif2a-left TCACCTACAGAAAGTGTC 15 ZebrafishCommunity-eif2a-right TGCTCGGCTGTGATCCAG 16 ZebrafishCommunity-eomesb-left TCTTCTCACCCGGAGGCG 16 ZebrafishCommunity-eomesb-right TCACCACAAACTACCGGA 17 ZebrafishCommunity-epd-left TGGGCTTCTCATCACTCT 17 ZebrafishCommunity-epd-right TGGTCAGCTGTGGTGAAT 18 ZebrafishCommunity-f11r-left TTGACTTGCAATGTTCCT 18 ZebrafishCommunity-f11r-right TCTACTCTGGGTGTTGCT 19 ZebrafishCommunity-fabp3-left TGTTTGTCTTCTGTTGTT 19 ZebrafishCommunity-fabp3-right TCCCTCCATCTAAAGTTA 20 ZebrafishCommunity-fastkd3-left TACCTGTCTTCTAATGAG 20 ZebrafishCommunity-fastkd3-right TACAAGAGGAGAGGCGTT 21 ZebrafishCommunity-FoxF2a-left TCAGGTCCAGCCCGGCAT 21 ZebrafishCommunity-FoxF2a-right TGCGTGTTTTGGAGAGCC 22 ZebrafishCommunity-fshr-left TGTTGTCAATGATGCTGT 22 ZebrafishCommunity-fshr-right TCGGTGTTTGCCATGGAG 23 ZebrafishCommunity-GRIN2B-left TCCAGGTGTACCCGTACC 23 ZebrafishCommunity-GRIN2B-right TCATCTTCCAGGTGGCGC 24 ZebrafishCommunity-GRM2b-left TATCTTGCCCGGCTTGAA 24 ZebrafishCommunity-GRM2b-right TTTCGAGCAGGTGTCCAG 25 ZebrafishCommunity-hcrt-left TACAAGACAGGGGACAGA 25 ZebrafishCommunity-hcrt-right TCTTACCTTAGCTGTG 26 ZebrafishCommunity-hs3st3l-left TCACCTGCATCTCCATCC 26 ZebrafishCommunity-hs3st3l-right TCGGAGGAGTCGCAGCAT 27 ZebrafishCommunity-hsd17b1-left TTGTTCTCATCACTGGAT 27 ZebrafishCommunity-hsd17b1-right TGCACAGCAAGGCTGAGT 28 ZebrafishCommunity-Hsf1-left TCACCGGGAACAATGTGC 28 ZebrafishCommunity-Hsf1-right TCCACCAGCGTCCAGAGT 29 ZebrafishCommunity-igf3-left TGCCAGACAACACTGAAG 29 ZebrafishCommunity-igf3-right TCCACTAGTTCTCGTCCG 30 ZebrafishCommunity-igfbp1a-left TGCTTGCCGAAACCGGGA 30 ZebrafishCommunity-igfbp1a-right TGTCCCCGGGTGAGCGCG 31 ZebrafishCommunity-igfbp1b-left TCCGGGATCCGGGCTGCG 31 ZebrafishCommunity-igfbp1b-right TCGCCGCTCCGCAGGGCG 32 ZebrafishCommunity-Impacthomolog(mouse)-left TGGACAAATTAGAGGATC 32 ZebrafishCommunity-Impacthomolog(mouse)-right TCCTCTATCTGAGACTGC 33 ZebrafishCommunity-INF2-left TAAACGCCCTGATCTTCA 33 ZebrafishCommunity-INF2-right TGCGCATCTTGTCTCTCT 34 ZebrafishCommunity-inhibitorofDNAbinding3-left TCAACATTGGATATCTAT 34 ZebrafishCommunity-inhibitorofDNAbinding3-right TCTGGACACTGGGATAAA 35 ZebrafishCommunity-kcnma1a-left TGCCTGGCACAGGGCCTG 35 ZebrafishCommunity-kcnma1a-right TGACCTCATGGAGAAGAG 36 ZebrafishCommunity-kcnma1b-left TGCCTGGCACAGGGGCT 36 ZebrafishCommunity-kcnma1b-right TCATTGAGAACAGGTT 37 ZebrafishCommunity-KISS1RA-left TTTTCTGGTGAAGACTGG 37 ZebrafishCommunity-KISS1RA-right TTCTGAAAGAGCCATTCA 38 ZebrafishCommunity-KISS1RB-left TGATGGCAGAAACTAACA 38 ZebrafishCommunity-KISS1RB-right TTGCACATGATGTGCTCT 39 ZebrafishCommunity-klf8-left TGAGCCCACAGACCTCAA 39 ZebrafishCommunity-klf8-right TGATGATGCAGAGCGGTG 40 ZebrafishCommunity-klf4a-left TGTCTCACCTGAAACGCC 40 ZebrafishCommunity-klf4a-right TGGTCAGCGCAGGTCGCA 41 ZebrafishCommunity-LBH-left TGTGCTCGTCTTCAGATC 41 ZebrafishCommunity-LBH-right TACAGCAGCGCTCGAAGT 42 ZebrafishCommunity-lycat-left TCCTTCATCTTCATCCAG 42 ZebrafishCommunity-lycat-right TGCTCATGTGTGTGCGGT 43 ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-left TGATCTGTTCCTGGTAGC 43 ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)- right TTCCAGTCCAAGGGCATG 44 ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)- left TGTTCCTGGTGGCGGTGC 44 ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)- right TGAGTGCTCCAAACCAAG 45 ZebrafishCommunity-mef2d-left TGGGGAGGAAAAAGATCC 45 ZebrafishCommunity-mef2d-right TGTCTGTTACGCTCGTCT 46 ZebrafishCommunity-meis2b-left TGCTGGCGCTGGTGTTTG 46 ZebrafishCommunity-meis2b-right TCTCTCGGTGTGCATGTG 47 ZebrafishCommunity-mical2b-left TGGGGGAGACGGAGGAAG 47 ZebrafishCommunity-mical2b-right TTCTCAAAAAGCTGACCG 48 ZebrafishCommunity-mtdhb-left TGCCAGCACAATAGATTG 48 ZebrafishCommunity-mtdhb-right TGCGACGCGATCTCTTGC 49 ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-left TGCCGCCAACTGGCTGAA 49 ZebrafishCommunity-NADPHoxidase_EF-handcalciumbindingdomain5-right TTTTGCGCTTGTTCTG 50 ZebrafishCommunity-Neurl1a-left TCTCTAGGTGGACCATTT 50 ZebrafishCommunity-Neurl1a-right TGGGCTTGTGATGGCATC 51 ZebrafishCommunity-NPHP5/iqcb1-left TGGTCTGGCTGCTTTCAG 51 ZebrafishCommunity-NPHP5/iqcb1-right TGGCAGAAATTCATGACT 52 ZebrafishCommunity-NR1I2-left TCGGAGCTCGCCGTGTCT 52 ZebrafishCommunity-NR1I2-right TAAGCGGGACATTGACGT 53 ZebrafishCommunity-pax1a-left TGAAAAACATACGGGACT 53 ZebrafishCommunity-pax1a-right TCCCATGCAAAAATTCCC 54 ZebrafishCommunity-pax1b-left TGGGAATCAGACCCTGCG 54 ZebrafishCommunity-pax1b-right TCCATGAGAGACCCTG 55 ZebrafishCommunity-pax7a-left TTAGAAGTCTCAACACCT 55 ZebrafishCommunity-pax7a-right TCCACCGAGTTGATTGA 56 ZebrafishCommunity-pbx2-left TGGAGCAGACCCCGCGGG 56 ZebrafishCommunity-pbx2-right TGCGGTTGTCGTGTCCGT 57 ZebrafishCommunity-pcdh10a-left TCGCCGAAGATCTGGGGC 57 ZebrafishCommunity-pcdh10a-right TGGAAACGGCGCGCGGAA 58 ZebrafishCommunity-pls1-left TACGGGAGATTGTAGAGA 58 ZebrafishCommunity-pls1-right TTCTCATCTTTGTTGGTG 59 ZebrafishCommunity-Prdx3-left TGCTTTCACGCATTGCAC 59 ZebrafishCommunity-Prdx3-right TCTCCCGATGGTGGCTGC 60 ZebrafishCommunity-prp19-left TTCCAGTTTCAAACGAAG 60 ZebrafishCommunity-prp19-right TTGGAAACAGGGGACACA 61 ZebrafishCommunity-prrx1a-left TGGGTTTCGTCAATTTGC 61 ZebrafishCommunity-prrx1a-right TGTCCATTACATGAGCAT 62 ZebrafishCommunity-rbfox2-left TGAGGAGGGCGCAGTGGT 62 ZebrafishCommunity-rbfox2-right TGGAGGAGGAGGAGGAGG 63 ZebrafishCommunity-rbp4-left TGTTAAGGCTCTGTATAG 63 ZebrafishCommunity-rbp4-right TCCTGAGCCCAGCAAGTG 64 ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive) -left TCTTTTTGTGCTGCGCCA 64 ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive) -right TTCATGCCTGTAGAAGAC 65 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorb-left TGGAGAAACAGAAGATGA 65 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorb-right TCCAAAAGTGAAGACAGC 66 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-left TAAGAGGGCGGAGCTTTT 66 ZebrafishCommunity-retinitispigmentosaGTPaseregulatorbORF15-right TCTAAGTAACTCAGTGGG 67 ZebrafishCommunity-sdc4-left TGGAGTCCTCCGGCAACT 67 ZebrafishCommunity-sdc4-right TTTAAGTCGGTGAACTCA 68 ZebrafishCommunity-sdcbp-left TCTCCCCGAACCTTCCAG 68 ZebrafishCommunity-sdcbp-right TGAATCATAGTAGGTACT 69 ZebrafishCommunity-sdccag8-left TGGTTCCCATCATAAACA 69 ZebrafishCommunity-sdccag8-right TCAGCCTCCAGGTGCTGA 70 ZebrafishCommunity-si:dkey-208k22.3-left TTATTCTCAGGCCAACTT 70 ZebrafishCommunity-si:dkey-208k22.3-right TTTCGACTTGTGCATCTT 71 ZebrafishCommunity-six4a-left TTTGTTTGCGCACAGTTC 71 ZebrafishCommunity-six4a-right TGAGAAAGCCAAAGAGGT 72 ZebrafishCommunity-slc17a9a-left TCTCCATTGGTCACGGAT 72 ZebrafishCommunity-slc17a9a-right TCTAGCCATGGTGACGGC 73 ZebrafishCommunity-slc17a9b-left TGCTCTACTGTGCGCGTG 73 ZebrafishCommunity-slc17a9b-right TCCGCCATGCTCACGGCA 74 ZebrafishCommunity-slit3-left TGGACAGGAATAACATCA 74 ZebrafishCommunity-slit3-right TTGAGGCCAGAGAAGTCC 75 ZebrafishCommunity-slmapb-left TGGAGGCCAGTGAGTCTG 75 ZebrafishCommunity-slmapb-right TGCAGTTCCTGGTTCAGC 76 ZebrafishCommunity-smyd1a-left TGGAGGTGTTCGGGGCCG 76 ZebrafishCommunity-smyd1a-right TCTCTGGTACCCCGCAGA 77 ZebrafishCommunity-snai1b-left TGAAGTTTCGAGGGGATA 77 ZebrafishCommunity-snai1b-right TCTTGACAAGAAATGAGC 78 ZebrafishCommunity-spermassociatedantigen7-left TTCTGGGCTCGATCTTGA 78 ZebrafishCommunity-spermassociatedantigen7-right TGATCGTGAACGGTTGGA 79 ZebrafishCommunity-sqstm1-left TGTCGATGACAGTGAAAG 79 ZebrafishCommunity-sqstm1-right TCCTTGTTGCAGTCCTCC 80 ZebrafishCommunity-srf-left TGGACGCTCTGGTAATGG 80 ZebrafishCommunity-srf-right TGTGCTGCCCACCTGTAG 81 ZebrafishCommunity-Stat3-left TGCAGCAGTTGGAGACGC 81 ZebrafishCommunity-Stat3-right TCGCTGTACAGGTGATAC 82 ZebrafishCommunity-stxbp1a-left TCAGCTTGCCCTGACCCC 82 ZebrafishCommunity-stxbp1a-right TGGAGGCACGGCTCTTCA 83 ZebrafishCommunity-Sun1-left TGGATTTTTCCCGTCTGC 83 ZebrafishCommunity-Sun1-right TTATCCGGAGTACAGTGA 84 ZebrafishCommunity-swap70b-left TAAGGGACGAGCTTCTC 84 ZebrafishCommunity-swap70b-right TCCAGGGCTGTGAAGG 85 ZebrafishCommunity-SYNE2-left TATGGGATGAGCTGGACC 85 ZebrafishCommunity-SYNE2-right TCGCCCTCTAACTCAGCT 86 ZebrafishCommunity-tbx4-left TGCTTCAGGAGAAGGCCT 86 ZebrafishCommunity-tbx4-right TGGGCAACGGTCATTCCT 87 ZebrafishCommunity-tetmethylcytosinedioxygenase1(previous-left TCAACTCGCGCATCCACA 87 ZebrafishCommunity-tetmethylcytosinedioxygenase1(previous-right TCATGGAAGCCTTCACCC 88 ZebrafishCommunity-tetmethylcytosinedioxygenase3-left TTATTAGGCACTTTTTTG 88 ZebrafishCommunity-tetmethylcytosinedioxygenase3-right TGTGGACAGGAAAGTGCA 89 ZebrafishCommunity- tetmethylcytosinedioxygenase3(previoussi:ch211-220a11.1)-left TCAAATCCTGAATCTCAA 89 ZebrafishCommunity- tetmethylcytosinedioxygenase3(previoussi:ch211-220a11.1)-right TTGTGCTGGCATTTTGAC 90 ZebrafishCommunity-TIMM22-left TTGTTCTGGGAGGTGCT 90 ZebrafishCommunity-TIMM22-right TTTGCATCAATGCCGG 91 ZebrafishCommunity-tppp3-left TGAGGGTTCGGGTGAAAT 91 ZebrafishCommunity-tppp3-right TTTACGAAATGCCATCTC 92 ZebrafishCommunity-trappc11-left TCCTATGAGTGGTACATT 92 ZebrafishCommunity-trappc11-right TGTTCATCCAGCCCGTCT 93 ZebrafishCommunity-ucma-left TGCTGATCGCGCTGACTG 93 ZebrafishCommunity-ucma-right TTTTCCATCTTTAACA 94 ZebrafishCommunity-vclbAL772263.1-left TGGCCCAGCAGATCTCCC 94 ZebrafishCommunity-vclbAL772263.1-right TCCACCTCTCCCTCCTCG 95 ZebrafishCommunity-ybx1-left TGAGCAGCGAGGCCGAGA 95 ZebrafishCommunity-ybx1-right TCCGCGTCGGCGGCAGGC 96 ZebrafishCommunity-yjefn3-left TTGAGACGGAGCTCCTGA 96 ZebrafishCommunity-yjefn3-right TCAATCAGCTGCTGCTGG
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