🚀 go-pugleaf

--Apple-Mail=_9F0A136E-C538-4C15-9C78-E701C3A12D6B
Content-Transfer-Encoding: 7bit
Content-Type: text/plain;
	charset=us-ascii

Dear Colleagues,

I'm pleased to provide an update on our construction of engineered
TALENs for our FOURTH NIH-sponsored solicitation of zebrafish gene
targets.  We now have sequence-verified plasmids encoding TALEN pairs
for 94 of the 96 genes we targeted.  The names of these 94 genes are
listed below.  We are still awaiting plasmids for the NPVF and oxtr
genes and hope to provide an update on these shortly.  All of these
plasmids will be deposited with Addgene in the coming days and, after
they pass Addgene's quality control checks, will be made available
here:  http://www.addgene.org/talengineering/TALENzebrafish/.  The
deposit of these new TALEN pairs will bring the total number of
zebrafish genes targeted with engineered nucleases by this effort to
nearly 250.

An announcement regarding the next 96 genes to be targeted in our
FIFTH solicitation will be made in the coming days.

With best regards,

Keith Joung
Randy Peterson
Joanna Yeh
Jon Foley
Deepak Reyon
Jeff Sander

Massachusetts General Hospital

94 new genes for which TALENs have been completed:

aak1(1of2)
amh
arf6
arnt1
atg12
atg5
avpl
Ccl2
cebpb
CIT1
cited2
ComplementFactorH
crispld2
dachc
dbx1b
DGKE
Dlk1
Dlk2
drd4b
EMP2
eps15
esr1
fam123b
fgfbp1a
foxl2
FoxP1b
foxp3a
Frizzled5
Fryl
fscn2a
GnIH
GnRH3
gper
hemogen-site1
hemogen-site2
hif1al-site1
hif1al-site2
hirip3
hist2h3ca1
hnrnpr
hnrnpu
hs3st1
hs3st1l1
Id4
IPIP27A
jarid2a
lhx1a
lrrtm1
mak
mapk14a
max
mchr1a
mef2aa
meis2a
meis4.1a
mrc1a
nr5a2
nup155
NUPL2
ovol1
oxtrl
park2
pcolce2b
pink1
Popdc2
Popdc3
prothymosinalphaa
prrx1b
PurinergicreceptorY2
rassf7b
scrt2
sema6dl
Semaphorin6bb
si:ch211-120j21.1
slc50a1
snrnp35
snrnp48
sox1a
Sox2
Tac3b
taok2b
tcof
tet2(ENSDART00000113020)
tet3(ENSDART00000137355)
tfe3a
tfe3b
tnfb
TRABD2A
TTC19
Vangl1
vasa
Vrk2
ywhai
zmynd11
--Apple-Mail=_9F0A136E-C538-4C15-9C78-E701C3A12D6B
Content-Transfer-Encoding: quoted-printable
Content-Type: text/html;
	charset=us-ascii

<html><head></head><body style=3D"word-wrap: break-word; =
-webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear =
Colleagues,<br><br>I'm pleased to provide an update on our construction =
of engineered<br>TALENs for our FOURTH NIH-sponsored solicitation of =
zebrafish gene<br>targets. &nbsp;We now have sequence-verified plasmids =
encoding TALEN pairs<br>for 94 of the 96 genes we targeted. &nbsp;The =
names of these 94 genes are<br>listed below. &nbsp;We are still awaiting =
plasmids for the NPVF and oxtr<br>genes and hope to provide an update on =
these shortly. &nbsp;All of these<br>plasmids will be deposited with =
Addgene in the coming days and, after<br>they pass Addgene's quality =
control checks, will be made available<br>here: &nbsp;<a =
href=3D"http://www.addgene.org/talengineering/TALENzebrafish/">http://www.=
addgene.org/talengineering/TALENzebrafish/</a>. &nbsp;The<br>deposit of =
these new TALEN pairs will bring the total number of<br>zebrafish genes =
targeted with engineered nucleases by this effort to<br>nearly =
250.<br><br>An announcement regarding the next 96 genes to be targeted =
in our<br>FIFTH solicitation will be made in the coming =
days.<br><br>With best regards,<br><br>Keith Joung<br>Randy =
Peterson<br>Joanna Yeh<br>Jon Foley<br>Deepak Reyon<br>Jeff =
Sander<br><br>Massachusetts General Hospital<br><br>94 new genes for =
which TALENs have been =
completed:<br><br>aak1(1of2)<br>amh<br>arf6<br>arnt1<br>atg12<br>atg5<br>a=
vpl<br>Ccl2<br>cebpb<br>CIT1<br>cited2<br>ComplementFactorH<br>crispld2<br=
>dachc<br>dbx1b<br>DGKE<br>Dlk1<br>Dlk2<br>drd4b<br>EMP2<br>eps15<br>esr1<=
br>fam123b<br>fgfbp1a<br>foxl2<br>FoxP1b<br>foxp3a<br>Frizzled5<br>Fryl<br=
>fscn2a<br>GnIH<br>GnRH3<br>gper<br>hemogen-site1<br>hemogen-site2<br>hif1=
al-site1<br>hif1al-site2<br>hirip3<br>hist2h3ca1<br>hnrnpr<br>hnrnpu<br>hs=
3st1<br>hs3st1l1<br>Id4<br>IPIP27A<br>jarid2a<br>lhx1a<br>lrrtm1<br>mak<br=
>mapk14a<br>max<br>mchr1a<br>mef2aa<br>meis2a<br>meis4.1a<br>mrc1a<br>nr5a=
2<br>nup155<br>NUPL2<br>ovol1<br>oxtrl<br>park2<br>pcolce2b<br>pink1<br>Po=
pdc2<br>Popdc3<br>prothymosinalphaa<br>prrx1b<br>PurinergicreceptorY2<br>r=
assf7b<br>scrt2<br>sema6dl<br>Semaphorin6bb<br>si:ch211-120j21.1<br>slc50a=
1<br>snrnp35<br>snrnp48<br>sox1a<br>Sox2<br>Tac3b<br>taok2b<br>tcof<br>tet=
2(ENSDART00000113020)<br>tet3(ENSDART00000137355)<br>tfe3a<br>tfe3b<br>tnf=
b<br>TRABD2A<br>TTC19<br>Vangl1<br>vasa<br>Vrk2<br>ywhai<br>zmynd11</body>=
</html>=

--Apple-Mail=_9F0A136E-C538-4C15-9C78-E701C3A12D6B--