Article View: bionet.software.srs
Article #88Re: using srs for Agrobacterium Tumefaciens c58
From: "benedict arnold (JIC)"
Date: Tue, 21 Sep 2004 17:44
Date: Tue, 21 Sep 2004 17:44
18 lines
3965 bytes
3965 bytes
Hi Jinal, > I tried downloading the protein sequences for the organism: Agrobacterium > Tumefaciens C58 strain and I found that there are around 10000 entries. I > remember that previously (before 1.5 months) when I tried the same query and > I got only 461 genes. My suspicion is that I am doing something wrong while > querying. Can you please let me know how shall I query the swissprot database > so that I can obtain genes for only "Agrobacterium Tumefaciens C58" organism. I think the problem here is that if you search only in the uniprot/swissprot databank you get 482 entries for the query, if you search in the uniprot/trembl databank you find 9812 entries, as data is moved from uniprot/trembl to uniprot/swissprot, I suspect what you've been doing is searching the uniprot/swissprot databank only and missed all the entires is the uniprot/trembl data. without knowing which SRS site you have been using, it might be that the site originally had swissprot meaning just the curated version) and sptrembl as separate databanks and they now have swissprot as a virtual library where both swissprot and sptrembl are combined, so whereas before you were searching a relatively small set of all the proteins out there you are now searching the whole set hope that helps Ben ---
Technical Details
Message-ID:
<286D8A554155B54CBFB18EF26EE2F1692780DD@jie2ksrv1.jic.bbsrc.ac.uk>Path:
arc1-ca.pugleaf.net!g2news1.google.com!news2.google.com!proxad.net!fr.ip.ndsoftware.net!216.196.110.149.MISMATCH!border2.nntp.ams.giganews.com!border1.nntp.ams.giganews.com!nntp.giganews.com!dedekind.zen.co.uk!demorgan.zen.co.uk!zen.net.uk!hamilton.zen.co.uk!193.60.199.26.MISMATCH!feed4.jnfs.ja.net!feed2.jnfs.ja.net!jnfs.ja.net!rfcgr.mrc.ac.uk!hgmp.mrc.ac.uk!net.bio.netShow Full Headers
Complete Article Headers
X-Google-Thread: fa6ec,dd31a0a57ba976f6,start
X-Google-Attributes: gidfa6ec,public
X-Google-Language: ENGLISH,ASCII-7-bit
Path: arc1-ca.pugleaf.net!g2news1.google.com!news2.google.com!proxad.net!fr.ip.ndsoftware.net!216.196.110.149.MISMATCH!border2.nntp.ams.giganews.com!border1.nntp.ams.giganews.com!nntp.giganews.com!dedekind.zen.co.uk!demorgan.zen.co.uk!zen.net.uk!hamilton.zen.co.uk!193.60.199.26.MISMATCH!feed4.jnfs.ja.net!feed2.jnfs.ja.net!jnfs.ja.net!rfcgr.mrc.ac.uk!hgmp.mrc.ac.uk!net.bio.net
From: benedict.arnold@bbsrc.ac.uk ("benedict arnold (JIC)")
Newsgroups: bionet.software.srs
Subject: Re: using srs for Agrobacterium Tumefaciens c58
Date: 21 Sep 2004 18:44:42 +0100
Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research
Lines: 19
Sender: mod-srs@rfcgr.mrc.ac.uk
Approved: BIOSCI Approval Key <biosci-approval-key@net.bio.net>
Message-ID: <286D8A554155B54CBFB18EF26EE2F1692780DD@jie2ksrv1.jic.bbsrc.ac.uk>
NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk
X-Trace: helium.hgmp.mrc.ac.uk 1095788682 2715 193.62.192.80 (21 Sep 2004 17:44:42 GMT)
X-Complaints-To: news@net.bio.net
NNTP-Posting-Date: Tue, 21 Sep 2004 17:44:42 +0000 (UTC)
X-Original-To: bionet-software-srs@net.bio.net
content-class: urn:content-classes:message
charset="iso-8859-1"
X-Original-To: bio-srs@net.bio.net
X-Received: from localhost (localhost [127.0.0.1])
by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DCBA87D10E
for <bio-srs@net.bio.net>; Thu, 9 Sep 2004 10:15:09 +0100 (BST)
X-Received: from mhub2.bbsrc.ac.uk (mhub2.bbsrc.ac.uk [149.155.202.2])
by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id F0C057D10B
for <bio-srs@net.bio.net>; Thu, 9 Sep 2004 10:15:06 +0100 (BST)
X-Received: from cse2ksrv1.acs.bbsrc.ac.uk ([149.155.100.84])
by mhub2.bbsrc.ac.uk with esmtp (Exim 4.30)
id 1C5L1D-000449-FY; Thu, 09 Sep 2004 10:15:03 +0100
X-Received: from jie2ksrv1.jic.bbsrc.ac.uk ([149.155.120.103]) by cse2ksrv1.acs.bbsrc.ac.uk with Microsoft SMTPSVC(5.0.2195.6713);
Thu, 9 Sep 2004 10:15:03 +0100
X-MimeOLE: Produced By Microsoft Exchange V6.0.6375.0
X-Thread-Topic: Re: using srs for Agrobacterium Tumefaciens c58
X-Thread-Index: AcSWTX0UYxDrxX/YRj2vAggCqYYQfg==
X-To: <bio-srs@net.bio.net>
X-Cc: <jjhaveri@gmail.com>
X-OriginalArrivalTime: 09 Sep 2004 09:15:03.0862 (UTC) FILETIME=[7DB10D60:01C4964D]
X-ECS-MailScanner-BBSRC: Found to be clean
X-Auth: PGPMoose V1.1 PGP bionet.software.srs
iQCVAwUAQVBoiaCIqsWvyF5FAQHYkgP+LlhbVvVtHAzQ46agQfwpVp78FyBgMbxI
4gbydXw2/L9Dalpvm+cTDA+LiSgymc30aMZY8ZvP+MYLKHQT/b3SDh/YQkyebX7o
g2DnMyt2Zbr3N/jXINwKjhQGcKp/bgzZP0weYeWYwr2c0Dtf002E6Cwb6ihmIxMI
HFmkZo8hZYo=
=WeYg