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Article #23975need prg to estimate band sizes on Southern's
From: danielg
Date: Thu, 14 Jun 1990 18:25
Date: Thu, 14 Jun 1990 18:25
26 lines
1946 bytes
1946 bytes
Am posting for a friend. He needs a program that he can run on his pc to estimate band sizes on Southern blots. His description was sketchy, bear with me. Currently, he uses a prg written in quick basic, in conjunction with a digitizing pad. His program works like this. Put the southern blot on the pad (visualization done by biotin labelling), and then he touches the pointer to the origin in lane #1, then on each band in lane #1, which is MW markers. He enters the band sizes, and the prg figures a std curve, basically a plot of fragment size vs. migration distance. From this, he can estimate band sizes in the following lanes. His problem is that he loses accuracy with fragments larger than 7 kb. Anyone familiar with any programs that he can get? I know that there are some nice *expensive* machines that do this, so if any of you have any info or experience using them, your input is welcome as well. Hopefully, dan __________________________________________________________________________ **** The shallow man has *** | Do not be overly righteous, nor overly **** has opinions; *** | wise: why should you destroy yourself ? **** the deep, *** | - King Solomon, wisest man of his **** convictions. *** | day, Ecclesiastes 7:16.
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: fa6b6,d028f2c55beb1712,start X-Google-Attributes: gidfa6b6,public X-Google-ArrivalTime: 1990-06-14 19:49:05 PST Path: arc1-ca.pugleaf.net!gmdzi!unido!mcsun!uunet!snorkelwacker!bu.edu!ncsuvx!mcnc!ecsgate!uncmed!uncmed.med.unc.edu!danielg From: danielg@uncmed.med.unc.edu Newsgroups: bionet.software Subject: need prg to estimate band sizes on Southern's Message-ID: <316@uncmed.med.unc.edu> Date: 14 Jun 90 18:25:39 GMT Sender: news@uncmed.med.unc.edu Reply-To: danielg@uncmed.med.unc.edu () Organization: UNC-CH School of Medicine Lines: 26 Posted: Thu Jun 14 19:25:39 1990