🚀 go-pugleaf

RetroBBS NetNews Server

Inspired by RockSolid Light RIP Retro Guy

Article View: bionet.software
Article #23975

need prg to estimate band sizes on Southern's

#23975
From: danielg
Date: Thu, 14 Jun 1990 18:25
26 lines
1946 bytes
Am posting for a friend.  He needs a program that he can run on his pc to
estimate band sizes on Southern blots.  His description was sketchy, bear
with me.

Currently, he uses a prg written in quick basic, in conjunction with a
digitizing pad.  His program works like this.  Put the southern blot on
the pad (visualization done by biotin labelling), and then he touches the
pointer to the origin in lane #1, then on each band in lane #1, which is
MW markers.  He enters the band sizes, and the prg figures a std curve,
basically a plot of fragment size vs. migration distance.  From this, he
can estimate band sizes in the following lanes.

His problem is that he loses accuracy with fragments larger than 7 kb.

Anyone familiar with any programs that he can get?  I know that there are
some nice *expensive* machines that do this, so if any of you have any
info or experience using them, your input is welcome as well.

Hopefully,

dan
__________________________________________________________________________
 **** The shallow man has *** |  Do not be overly righteous, nor overly
 **** has opinions;       *** |  wise: why should you destroy yourself ?
 **** the deep,           *** |      - King Solomon, wisest man of his 
 **** convictions.        *** |        day, Ecclesiastes 7:16.
Technical Details
Message-ID: <316@uncmed.med.unc.edu>
Path: arc1-ca.pugleaf.net!gmdzi!unido!mcsun!uunet!snorkelwacker!bu.edu!ncsuvx!mcnc!ecsgate!uncmed!uncmed.med.unc.edu!danielg

Show Full Headers
Complete Article Headers
X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: fa6b6,d028f2c55beb1712,start
X-Google-Attributes: gidfa6b6,public
X-Google-ArrivalTime: 1990-06-14 19:49:05 PST
Path: arc1-ca.pugleaf.net!gmdzi!unido!mcsun!uunet!snorkelwacker!bu.edu!ncsuvx!mcnc!ecsgate!uncmed!uncmed.med.unc.edu!danielg
From: danielg@uncmed.med.unc.edu
Newsgroups: bionet.software
Subject: need prg to estimate band sizes on Southern's
Message-ID: <316@uncmed.med.unc.edu>
Date: 14 Jun 90 18:25:39 GMT
Sender: news@uncmed.med.unc.edu
Reply-To: danielg@uncmed.med.unc.edu ()
Organization: UNC-CH School of Medicine
Lines: 26
Posted: Thu Jun 14 19:25:39 1990