Oligos

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Article #24055

#24055

From: Gary Williams x3294
Date: Wed, 31 Jan 1990 15:19

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Does anyone know of a program to aid the selection of regions of a
nucleic acid sequence to act as good primers for PCR?

Ideally, the regions required should have a high Td so that they do not
anneal to themselves.  They should form stable duplexes with and be
specific to, the target sequence.  They should not base-pair to other
regions on the target sequence.


Gary Williams

Computing Services Section,		 Janet:       G.Williams@UK.AC.CRC
MRC-Clinical Research Centre,		 Elsewhere:   G.Williams@CRC.AC.UK
Watford Rd, HARROW, Middx, HA1 3UJ, U.K. EARN/Bitnet: G.Williams%CRC@UKACRL
Tel 01-869 3294    Fax 01-423 1275       Usenet: ...!mcvax!ukc!mrccrc!G.Williams

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From: gwilliam@mrc-crc.ac.uk (Gary Williams x3294)
Newsgroups: bionet.software
Subject: Oligos
Message-ID: <9001311519.AA09451@uk.ac.crc>
Date: 31 Jan 90 15:19:03 GMT
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Posted: Wed Jan 31 16:19:03 1990