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Article #2445MIRA: 454 reduced representation library
From: wodans
Date: Mon, 31 May 2010 08:18
Date: Mon, 31 May 2010 08:18
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3924 bytes
Hi All, I've got 454 sequences from a reduce representation library (restriction enzyme digested, the area from ~600-900 bp was extracted and used for the 454 without mechanically shearing the DNA)). Hence, I do not expect that the contigs should be very long and I therefore used the EST setting when assembling the sequences as to me the reduced representation data would look similar to EST, and got some 75K contigs (which to me makes sense). However, I also used the genome setting and got an order of magnitude fewer contigs... Would anyone know if I'm wrong using the EST settings? Thanks
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