N15 Leu Specific labeling

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1 total messages Started by Ramakrishna V. Thu, 20 Jan 2000 00:00

#259

Author: Ramakrishna V.
Date: Thu, 20 Jan 2000 00:00

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dear netters,
i would like to know the effect of using BL21/DE3 E.coli strain for
specifically labeling Leucine residues. I am interested in knowing
to what other amino acids will the label be scambled. any information
in this regard will be greatly appreciated.

thanking you in advance
ram

PS:  i have come across a paper in which N15 Leu labeling was performed
using E.Coli JM107 strain. in this case the label was scrambled to VAL
and ILE and to a lesser extent ALA. does the scambling of label depend
on the strain used?

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