Use of Value of TCID50 result

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Article #331

#331

From: masud
Date: Wed, 16 May 2012 15:57

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I have measured TCID50 value of virus: 1.6X10^4/ml. Now how do I use
the value in the real infection assay? If the infection fianl volume
is 100 microliter, for 1 TCID50 (of above value) or 100 TCID50 (of the
same virus stock) what volume we need to infect? Does the cell number
in 100 microliter is a concern or not? If any body can discuss.
Thanks.

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From: masud <masud1234@yahoo.com>
Date: Wed, 16 May 2012 08:57:38 -0700 (PDT)
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