Determination of particle/pfu ratio

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3 total messages Started by ukats Mon, 17 Mar 2008 21:21

#129

Author: ukats
Date: Mon, 17 Mar 2008 21:21

8 lines
2096 bytes

Dear all,

I have OD 260 value and PFU/ml value of purified virus
sample. How then do I calculate particle/pfu ratio
using these values?

Thanks,
Ukats.

Re: Determination of particle/pfu ratio

#130

Author: bob
Date: Sat, 22 Mar 2008 16:18

16 lines
1887 bytes

On Mon, 17 Mar 2008 14:21:36 -0700 (PDT), ukats <ukats02@yahoo.com>
wrote:

>Dear all,
>
>I have OD 260 value and PFU/ml value of purified virus
>sample. How then do I calculate particle/pfu ratio
>using these values?
>

So, do you know how many virus particles corresponds to 1 OD unit?

If not, you will need to measure it. Electron microscopy? Or some
labeling.

bob

RE: [Virology] Re: Determination of particle/pfu ratio

#131

Author: Randhawa, Parmjeet
Date: Mon, 24 Mar 2008 18:10

43 lines
3438 bytes

If you know the size in base pairs of the virus, you can calculate the
molecular weight of the viral DNA (1 nucleotide PAIR= 660 daltons).
Viral DNA concentration (calculated from OD 260 value) can then be
converted to viral particles using Avogadro's number (1 Gram MW=
6.02e+23 viral particles).

Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237

-----Original Message-----
From: virology-bounces@oat.bio.indiana.edu
[mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of bob
Sent: Saturday, March 22, 2008 12:18 PM
To: bionet-virology@magpie.bio.indiana.edu
Subject: [Virology] Re: Determination of particle/pfu ratio

On Mon, 17 Mar 2008 14:21:36 -0700 (PDT), ukats <ukats02@yahoo.com>
wrote:

>Dear all,
>
>I have OD 260 value and PFU/ml value of purified virus
>sample. How then do I calculate particle/pfu ratio
>using these values?
>

So, do you know how many virus particles corresponds to 1 OD unit?

If not, you will need to measure it. Electron microscopy? Or some
labeling.

bob

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