[Virology] Calculating virus quantities and ratios

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1 total messages Started by jirasu Tue, 11 Jan 2011 22:57

#325

Author: jirasu
Date: Tue, 11 Jan 2011 22:57

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I don´t know how to calculate a prosentage of viruses we managed to get from
our specimen,compared to the amount from the viruses that were put to the
specimen in the first place (% of the amount of viruses in the positive
RNA-control). And what were the amounts of viruses in PCR-units.
I´m quite desperate cause if I won´t get this done I can´t do the final
examn.
We put only 5ul of specimen in the DNA-strip when in the end the whole
amount of the separated RNA was 60ul. 

Quantitation Report:

Positive RNA-control
   Ct-value 24,88
   Calc conc (copies) 8826
1 sample
   Ct-value 28,99
   Calc conc 527
2 sample 
   Ct-value 32,15
   Calc conc 60





I´m grateful for any answere!
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