Re: Visible DNA stain

Article View: bit.listserv.confocal

Article #102

Back to bit.listserv.confocal Previous Next

#102

From: G. Macdonald
Date: Fri, 17 Mar 2000 00:00

20 lines
1415 bytes

The degree of RNA signal will partly depend upon the amount of RNA in the
cell, of course.  Diluting the dye may drop theRNA labelling to an
insignificant level.  The green Syto dyes from Molecular Probes are very
bright, and in some neurons stain solely the nucleus and nucleolus when
diluted to 1/2000 or higher.  Some other neuron types are so loaded with
rough ER that a highly concentrated Syto 13 (1/500) made them stand out.

Regards,
Glen


Glen MacDonald
   Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA  98195-7923
(206) 616-4156
glenmac@u.washington.edu

Technical Details

Message-ID: <Pine.A41.4.10.10003171014500.56038-100000@homer16.u.washington.edu>

Path: arc1-ca.pugleaf.net!unknown!not-for-mail

References: <20000317152843.8217.qmail@web803.mail.yahoo.com>

Show Full Headers

Complete Article Headers

X-Google-Language: ENGLISH,ASCII-7-bit
X-Google-Thread: 114d69,a5700b26d89b9df6
X-Google-Attributes: gid114d69,public
From: "G. Macdonald" <glenmac@U.WASHINGTON.EDU>
Subject: Re: Visible DNA stain
Date: 2000/03/17
Message-ID: <Pine.A41.4.10.10003171014500.56038-100000@homer16.u.washington.edu>
X-Deja-AN: 598850295
Sender: Confocal Microscopy List <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU>
References: <20000317152843.8217.qmail@web803.mail.yahoo.com>
Content-Type: TEXT/PLAIN; charset=US-ASCII
X-Trace: 17 Mar 2000 13:21:42 -0500, 127.0.0.1
X-Gateway-From: add_from_switch_to_drobot
MIME-Version: 1.0
Reply-To: Confocal Microscopy List <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU>
Newsgroups: bit.listserv.confocal

Reply to Parent Message