Re: Visible DNA Stain, Hoechts or DAPI?

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#113

From: Dr. Gerald Pfister
Date: Mon, 20 Mar 2000 00:00

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Dear all,

I'd like to add one remark to the discussion about toxicity of DAPI.

In a previous work I labeled different species of living ciliates with
DAPI to obtain labeled, but living prey organisms in protozoan feeding
experiments. The DAPI concentration for the live-stain was kept low
(when I remember right about 0.08%) for a staining time of an hour. This
stain lead to fluorescently labeled, living (and still reproducing!)
ciliates, which survived the stain without visible effects on their
condition for several weeks.
Due to these results, I think in low concentrations, DAPI cannot be that
toxic...

Who is interested in more detailed information, the mentioned results
are published:

Pfister & Arndt (1998): Food selectivity and feeding behaviour in
omnivorous filter-feeding ciliates: A case study for Stylonychia. Europ.
J. Protistol. 34, 446-457.

Kind regards,
Gerald.

    
-- 
Dr. Gerald Pfister
Institute for Biomedical Aging Research
Austrian Academy of Sciences
Rennweg 10
A - 6020 Innsbruck
Austria / Europe

Tel. +43-(0)512-583919-18
Fax: +43-(0)512-583919-8
email: gerald.pfister@oeaw.ac.at
http://www.oeaw.ac.at/iba/

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From: "Dr. Gerald Pfister" <Gerald.Pfister@oeaw.ac.at>
Subject: Re: Visible DNA Stain, Hoechts or DAPI?
Date: 2000/03/20
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