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Article #123Re: Plant cell wall
From: Kris.Vissenberg
Date: Wed, 31 May 2000 00:00
Date: Wed, 31 May 2000 00:00
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2292 bytes
Dear Dr. Taylor, I can recommend you the dye congo red which can be excited with the 488 or 514 nm laser lines. The dichroic properties of the dye can be used to visualize the mean cellulose microfibril orientation in plant cell walls as discussed in Verbelen and Stickens (1995) In vivo determination of fibril orientation in plant cell walls with polarization CLSM. J Microsc. 177, 1-6 Verbelen and Kerstens (2000) Polarization confocal microscopy and congo red fluorescence: a simple and rapid method to determine the mean cellulose firbil orientation in plants. J. Microsc. 198, 101-107. Best wishes, Kris --------------------------------------------------------------------------- Kris Vissenberg _/ _/ _/ _/_/_/_/ PhD-Student _/ _/ _/ _/ Dept.Biology (C 0.24) _/ _/ _/ _/ _/ Plant Physiology / Morphology _/ _/ _/ _/ _/ University of Antwerp (UIA) _/ _/ _/ _/_/_/_/_/ Universiteitsplein 1 _/ _/ _/ _/ _/ B - 2610 Wilrijk (Antwerpen) _/ _/ _/ _/ _/ Belgium _/_/_/_/ _/ _/ _/ Phone :+32-3-820.22.58 Fax n°:+32-3-820.22.71 E-mail:vissenbe@uia.ua.ac.be ---------------------------------------------------------------------------
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X-Google-Language: ENGLISH,ASCII X-Google-Thread: 114d69,2e00d73a1f9be319,start X-Google-Attributes: gid114d69,public From: "Kris.Vissenberg" <vissenbe@uia.ua.ac.be> Subject: Re: Plant cell wall Date: 2000/05/31 Message-ID: <Pine.OSF.4.21.0005311042100.25915-100000@hgins.uia.ac.be> X-Deja-AN: 629372443 Content-Transfer-Encoding: QUOTED-PRINTABLE References: <3.0.5.32.20000531083138.0369ee40@pophost.aber.ac.uk> X-Original-NNTP-Posting-Host: hgins.uia.ac.be To: Confocal Microscopy List <CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU> X-Sender: vissenbe@hgins.uia.ac.be Content-Type: TEXT/PLAIN; charset=ISO-8859-1 X-Complaints-To: abuse@belnet.be X-Trace: naxos.belnet.be 959762877 25073 143.169.254.1 (31 May 2000 08:47:57 GMT) Organization: University of Antwerp - UIA Mime-Version: 1.0 NNTP-Posting-Date: 31 May 2000 08:47:57 GMT Newsgroups: bit.listserv.confocal