🚀 go-pugleaf

I routinely use Cy 5 bought from Jackson lab and store it in glycerol as
per the instructions from jackson lab. However, I store it in -20C (not
frost-free) and it gives me very good results over many months.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro@nmsu.edu
http://confocal.nmsu.edu/eml

On Fri, 11 Aug 2000, Tom Phillips wrote:

> The other big problem with -20 freezers is that they so often are
> "frost-free" these days.  The auto defrost cycles on these types of
> freezers are very tough on antibodies and sensitive enzymes.  I
> believe Jackson recommends diluting their antibodies in 50% glycerol
> if you want to store at -20 so that they don't "freeze" but yet are
> kept cold.  I don't know if that limits the damage by a frost-free
> freezer but do know that the molecular biology types all stress that
> their enzymes diluted in glycerol are still very labile if stored in
> a frost-free freezer.
> 
> Tom
> 
> 
> >Tom Phillips wrote:
> >>
> >>  I was surprised by Martin's experience.  I buy lots of Jackson
> >>  antibodies (FITC, Cy3, Cy5, HRP; goat and donkey anti-mouse and
> >>  anti-rabbit) and generally aliquot them out as 10 ul/tube and freeze
> >>  and store at -80.  Once thawed, I dilute and use that day and throw
> >>  any extra away.  They have been stable for years this way.  Tom
> >
> >The differences in our experiences might have to do with our respective
> >speeds of freezing: -80 vs -20 (which was what I tried); 10 ul vs ~100
> >ul (which I used).  Faster freezing is better, in my
> >experience--probably allows less time for the liquid to partition into
> >salt-rich and salt-poor fractions.
> >--
> >Martin Wessendorf, Ph.D.                        office: (612) 626 0145
> >Associate Professor                             lab:    (612) 624 2991
> >Dept. Neuroscience                      Preferred FAX:  (612) 624 8118
> >University of Minnesota                 Dept FAX:       (612) 626 5009
> >Minneapolis, MN  55455                e-mail: martinw@mail.ahc.umn.edu
> 
> --
> Thomas E. Phillips, Ph.D.
> Associate Professor of Biological Sciences
> Director, Molecular Cytology Core Facility
> 
> 3 Tucker Hall
> Division of Biological Sciences
> University of Missouri
> Columbia, MO 65211-7400
> (573)-882-4712 (voice)
> (573)-882-0123 (fax)
>