🚀 go-pugleaf

Anda,

wheras I never had problems with overnight fixation of cells in 1.6 %
formaldehyde, I did have problems with the nailpolish: I have tried several
ones and they all killed the GFP before the slide was even on the scope. I have
heard rumors of nailpolish that does not harm GFP, but was never able to hunt
one down, therefore PLEASE:
Could you let us know the exact brand name, product number or whatever is
usefull to find the nailpolish you are using?

Thanks!  Steffen

--- Anda Cornea <corneaan@OHSU.EDU> schrieb:
>
> >>> "Goodhouse, Joseph" Long
> For fixation employ low percentage paraformaldehyde for the shortest periods
> of fixation as possible( 5- 10 minutes for cells).  Samples should be
> mounted in glycerol based mounting medium.  I have found 90% buffered
> glycerol to be best.  Avoid using nail polish as sealant of coverslips.  In
> a short time this will wipe out your GFP signal.
>
> We routinely use 4%paraformaldehyde, 30minutes fixation, for cells expressing
> a GFP-membrane receptor chimera and seal slides with nailpolish.  We see no
> loss of intensity when compared with live cells expressing the same
> construct, not even after a few months storage.
>

=====
Dr. Steffen Dietzel
Dept. of Cell and Structural Biology
University of Illinois at Urbana-Champaign
601 S. Goodwin Ave.
Urbana, IL 61801
Phone: +1/217/333-8372  Fax: +1/217/244-1648
e-mail: dietzels@yahoo.de  Web:  http://www.life.uiuc.edu/belmont/dietzel
__________________________________________________________________
Do You Yahoo!?
Gesendet von Yahoo! Mail - http://mail.yahoo.de
Yahoo! Auktionen - gleich ausprobieren - http://auktionen.yahoo.de